caa a22 4500 397552963 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00306.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00306.x Spectrochromatography of photosynthetic pigments as a fingerprinting technique for microbial phototrophs [Elektronische Daten] [Niels-Ulrik Frigaard, Kim L. Larsen, Raymond P. Cox] The combination of chromatographic separation using reverse-phase high-performance liquid chromatography and continuous monitoring of the column eluate using a diode-array spectrophotometer allowed qualitative and quantitative pigment profiling of extracts of photosynthetic material in a single run. Carotenoids and the spectrally distinct types of chlorophyll and bacteriochlorophyll can be unambiguously identified even when imperfectly separated on the column. The resulting spectrochromatogram is a fingerprint useful for the rapid characterization of pure cultures or mixed populations. We have developed software to allow recording, manipulation, and presentation of the resulting spectrochromatograms and present results from photosynthetic microbes in pure and mixed cultures. We describe a number of approaches to the presentation of the resulting data in a readily comprehensible form. © 1996 Federation of European Microbiological Societies. All rights reserved High-performance liquid chromatography nationallicence Diode-array spectrophotometry nationallicence Fingerprint nationallicence Pigment nationallicence Microbial phototroph nationallicence Frigaard Niels-Ulrik Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark aut Larsen Kim L. Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark aut Cox Raymond P. Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 69-77 20:2<69 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00306.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00306.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frigaard Niels-Ulrik Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark aut NATIONALLICENCE 700 1- Larsen Kim L. Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark aut NATIONALLICENCE 700 1- Cox Raymond P. Institute of Biochemistry, Odense University, Campusvej 55, 5230 Odense M, Denmark aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 69-77 20:2<69 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford