caa a22 4500 397552971 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00310.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00310.x Short exposure to acetylene to distinguish between nitrifier and denitrifier nitrous oxide production in soil and sediment samples [Elektronische Daten] [Ronald A. Kester, Wietse de Boer, Hendrikus J. Laanbroek] The contribution of nitrifiers and denitrifiers to the nitrous oxide production in slurries of calcareous silt loam and river bank sediment at different oxygen concentrations was determined using acetylene as nitrification inhibitor. The addition of 10 Pa acetylene resulted in inhibition of nitrous oxide production at oxic conditions, but strongly enhanced the nitrous oxide production at oxygen-poor and anoxic conditions. Inhibition of nitrification by short exposure (1 to 24 h) to high concentrations of acetylene (100 Pa to 10 kPa) was tested using the same samples. After the removal of acetylene, nitrification was inhibited almost completely (82% to 89%) for at least 6 days whereas nitrous oxide reduction was restored within a day. It was concluded that the ‘short exposure' inhibition method resulted in adequate nitrification inhibition without repressing the nitrous oxide reduction in anoxic nitrate-rich microsites and that the method was suitable for assessing the nitrifier contribution to the nitrous oxide emission of intact soil and sediment cores. © 1996 Federation of European Microbiological Societies. All rights reserved Nitrous oxide emission nationallicence Nitrification nationallicence Denitrification nationallicence Soil nationallicence Sediment nationallicence Kester Ronald A. Department of Plant-Microorganism Interactions, Centre for Terrestrial Ecology, Netherlands Institute of Ecology, P.O. Box 40, 6666 ZG Heteren, The Netherlands aut de Boer Wietse Department of Plant-Microorganism Interactions, Centre for Terrestrial Ecology, Netherlands Institute of Ecology, P.O. Box 40, 6666 ZG Heteren, The Netherlands aut Laanbroek Hendrikus J. Department of Microbial Ecology, Centre for Limnology, Netherlands Institute of Ecology, Nieuwersluis, The Netherlands aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 111-120 20:2<111 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00310.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00310.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kester Ronald A. Department of Plant-Microorganism Interactions, Centre for Terrestrial Ecology, Netherlands Institute of Ecology, P.O. Box 40, 6666 ZG Heteren, The Netherlands aut NATIONALLICENCE 700 1- de Boer Wietse Department of Plant-Microorganism Interactions, Centre for Terrestrial Ecology, Netherlands Institute of Ecology, P.O. Box 40, 6666 ZG Heteren, The Netherlands aut NATIONALLICENCE 700 1- Laanbroek Hendrikus J. Department of Microbial Ecology, Centre for Limnology, Netherlands Institute of Ecology, Nieuwersluis, The Netherlands aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 111-120 20:2<111 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford