caa a22 4500 397552998 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00197.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00197.x Cultivation of nitrifying bacteria in the retentostat, a simple fermenter with internal biomass retention [Elektronische Daten] [W. Tappe, C. Tomaschewski, S. Rittershaus, J. Groeneweg] The retentostat was developed for long-term continuous, axenic cultivation of microorganisms at those low growth rates which prevail in most natural habitats and which cannot be established properly in chemostats. How a microbial population approaches ‘zero-growth' was studied in axenic cultures of Nitrosomonas europaea with complete biomass retention at 25°C and constant input of a nutrient solution containing ammonium (0.57 mM) as energy source. Since only cell-free filtrate left the reactor, biomass accumulated until a stable maximum of 2.7 × 109 cells ml−1 (398 mg l−1 dry matter) was reached after about 5 weeks. In this state, growth rate approached zero, and the ammonium input just met the substrate demand required for maintenance energy (1.43 μmol NH3-N mg dm−1 h−1). The potential of the retentostat for studying interactions between different microorganisms was demonstrated with a cascade of cultures of Nitrosomonas, Nitrobacter, and a denitrifying Pseudomonas. Thereby the ammonia was completely eliminated from artificial wastewater. © 1996 Federation of European Microbiological Societies. All rights reserved Biomass retention nationallicence Maintenance energy nationallicence Nitrosomonas europaea nationallicence Nitrobacter winogradskyi nationallicence Bioreactor cascade nationallicence Tappe W. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut Tomaschewski C. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut Rittershaus S. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut Groeneweg J. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 47-52 19:1<47 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00197.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00197.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tappe W. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut NATIONALLICENCE 700 1- Tomaschewski C. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut NATIONALLICENCE 700 1- Rittershaus S. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut NATIONALLICENCE 700 1- Groeneweg J. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 3, 52425 Jülich, Germany aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 47-52 19:1<47 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford