caa a22 4500 397553005 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00194.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00194.x High survival efficiency and ribosomal RNA decaying pattern of Desulfobacter latus, a highly specific acetate-utilizing organism, during starvation [Elektronische Daten] [Manabu Fukui, Yuichi Suwa, Yoshikuni Urushigawa] Exponentially grown Desulfobacter latus cells were transferred to anaerobically prepared minimum medium without a carbon or energy substrate and incubated under anaerobic conditions. Changes in 16S ribosomal RNA (rRNA) of individual cells and the viable fraction in a population were monitored. The cell preparation was stained with a phylogenetic DNA probe labelled with fluorescent dye and the fluorescence of each cell was determined with confocal scanning laser microscope. Viable cells were defined as those capable of reducing a tetrazolium salt (the INT method [1]). The viability of a Desulfobacter starvation culture decreased to 85% in 48 h, but further decrease was not observed during prolonged starvation. The mean amount of 16S rRNA in individual cells decreased exponentially for 48 h to 30% the mean value obtained for exponentially growing cells, but did not decrease by prolonged starvation. About 30% of the mean content of 16S rRNA in growing cells was found in the starved cell population, suggesting that most individual cells in the starved population were not metabolically active. The difference between gross pixel intensity of cells having <8% of 16S rRNA in growing cells and those with a negative control probe was not significant. Thus, non-viable cells may not show positive signals by phylogenetic staining. © 1996 Federation of European Microbiological Societies. All rights reserved Desulfobacter latus nationallicence Starvation nationallicence Phylogenetic staining nationallicence Cell respiration nationallicence 16S ribosomal RNA nationallicence Confocal laser scanning microscope nationallicence Fukui Manabu National Institute for Resources and Environment, AIST, MITI, Onogawa 16-3, Tsukuba, Ibaraki 305, Japan aut Suwa Yuichi National Institute for Resources and Environment, AIST, MITI, Onogawa 16-3, Tsukuba, Ibaraki 305, Japan aut Urushigawa Yoshikuni National Institute for Resources and Environment, AIST, MITI, Onogawa 16-3, Tsukuba, Ibaraki 305, Japan aut FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 17-25 19:1<17 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00194.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00194.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukui Manabu National Institute for Resources and Environment, AIST, MITI, Onogawa 16-3, Tsukuba, Ibaraki 305, Japan aut NATIONALLICENCE 700 1- Suwa Yuichi National Institute for Resources and Environment, AIST, MITI, Onogawa 16-3, Tsukuba, Ibaraki 305, Japan aut NATIONALLICENCE 700 1- Urushigawa Yoshikuni National Institute for Resources and Environment, AIST, MITI, Onogawa 16-3, Tsukuba, Ibaraki 305, Japan aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 17-25 19:1<17 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford