caa a22 4500 397553048 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00199.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00199.x Spread and evolution of natural plasmids harboring transposon Tn5 [Elektronische Daten] [Jesús Blázquez, Alfonso Navas, Pilar Gonzalo, José L. Martínez, Fernando Baquero] The presence of transposon Tn5 was studied in 730 Enterobacteriaceae strains from clinical and sewage origin. From these strains, twenty-five conjugative plasmids harboring transposon Tn5 were isolated. These plasmids were compared with pJR67 and pRYC119, the only previously studied plasmids harboring Tn5. A phylogenetic tree of the evolution of all different plasmids was proposed. Irrespective of their bacterial host and geographical place of isolation, some of the plasmids were shown to be identical. All of them can be included in only eight different prototypical plasmid species. Twenty-two plasmids (88%) carried an IncI1 incompatibility determinant as judged form DNA hybridization experiments. The presence of some other common resistance genes suggested that these plasmids are descendants of a common ancestor. These IncI1 plasmids could be grouped in six prototypical species. The results presented here suggest that Tn5 spread in nature may be dependent on the conjugative ability of the IncI plasmids harboring the transposon, rather than on the efficiency of Tn5 transposition between different replicons. © 1996 Federation of European Microbiological Societies. All rights reserved Transposon Tn5 nationallicence Spread of resistance nationallicence Plasmid evolution nationallicence Blázquez Jesús Servicio de Microbiología, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Ctra. Colmenar Km 9100, 28034-Madrid, Spain aut Navas Alfonso Centro de Ciencias Medioambientales CSIC, Serrano 115 dupdo, 28006-Madrid, Spain aut Gonzalo Pilar Departamento de Microbiología, Colegio Universitario de Soria, Soria, Spain aut Martínez José L. Servicio de Microbiología, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Ctra. Colmenar Km 9100, 28034-Madrid, Spain aut Baquero Fernando Servicio de Microbiología, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Ctra. Colmenar Km 9100, 28034-Madrid, Spain aut FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 63-71 19:1<63 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00199.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00199.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blázquez Jesús Servicio de Microbiología, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Ctra. Colmenar Km 9100, 28034-Madrid, Spain aut NATIONALLICENCE 700 1- Navas Alfonso Centro de Ciencias Medioambientales CSIC, Serrano 115 dupdo, 28006-Madrid, Spain aut NATIONALLICENCE 700 1- Gonzalo Pilar Departamento de Microbiología, Colegio Universitario de Soria, Soria, Spain aut NATIONALLICENCE 700 1- Martínez José L. Servicio de Microbiología, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Ctra. Colmenar Km 9100, 28034-Madrid, Spain aut NATIONALLICENCE 700 1- Baquero Fernando Servicio de Microbiología, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Ctra. Colmenar Km 9100, 28034-Madrid, Spain aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 63-71 19:1<63 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford