caa a22 4500 397553056 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00195.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00195.x Bonin Patricia Centre d'Océanologie de Marseille, URA 41, Campus de Luminy, case 901, 13288 Marseille Cedex 09, France Anaerobic nitrate reduction to ammonium in two strains isolated from coastal marine sediment: A dissimilatory pathway [Elektronische Daten] [Patricia Bonin] A total of 28 nitrate-reducing bacteria were isolated from marine sediment (Mediterranean coast of France) in which dissimilatory reduction of nitrate to ammonium (DRNA) was estimated as 80% of the overall nitrate consumption. Thirteen isolates were considered as denitrifiers and ten as dissimilatory ammonium producers. 15N ammonium production from 15N nitrate by an Enterobacter sp. and a Vibrio sp., the predominant bacteria involved in nitrate ammonification in marine sediment, was characterized in pure culture studies. For both strains studied, nitrate-limited culture (1 mM) produced ammonium as the main product of nitrate reduction (> 90%) while in the presence of 10 mM nitrate, nitrite was accumulated in the spent media and ammonia production was less efficient. Concomitantly with the dissimilation of nitrate to nitrite and ammonium the molar yield of growth on glucose increased. Metabolic products of glucose were investigated under different growth conditions. Under anaerobic conditions without nitrate, ethanol was formed as the main product; in the presence of nitrate, ethanol disappeared and acetate increased concomitantly with an increased amount of ammonium. These results indicate that nitrite reduction to ammonium allows NAD regeneration and ATP synthesis through acetate formation, instead of ethanol formation which was favoured in the absence of nitrate. © 1996 Federation of European Microbiological Societies. All rights reserved Nitrate reduction nationallicence Ammonium nationallicence Marine bacterium nationallicence Dissimilatory pathway nationallicence FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 27-38 19:1<27 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00195.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00195.x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Bonin Patricia Centre d'Océanologie de Marseille, URA 41, Campus de Luminy, case 901, 13288 Marseille Cedex 09, France NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/1(1996-01), 27-38 19:1<27 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford