caa a22 4500 397553064 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00331.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00331.x Comparative analysis of the genetic structure of a Rhizobium meliloti field population before and after environmental release of the highly competitive R. meliloti strain GR4 [Elektronische Daten] [Pablo J. Villadas, Pedro Burgos, Doris Jording, Werner Selbitschka, Alfred Pühler, Nicolás Toro] Rhizobium meliloti strain GR4, which exhibits a highly competitive ability for alfalfa root nodule occupancy, was used in a field release experiment in Granada, Spain. In order to analyze the ecological impact of the GR4 release, we characterized the R. meliloti indigenous population of the field site by ERIC-(enterobacterial repetitive intergenic consensus) PCR and IS (insertion sequence) fingerprinting. Both fingerprinting methods resulted in the same grouping of the isolates. Data obtained were compared with a previous analysis by plasmid based sequence-specific PCR. Isolates belonging to the major infective group, as defined by dominant plasmid types, were shown to have identical or nearly identical ERIC and IS fingerprint patterns. Hence, we conclude that all three typing methods are suited to characterize the genetic structure of the field population. The possible impact of the introduction of strain GR4 was examined two years after its release in its original environment. No effect on the genetic structure of the indigenous R. meliloti field population was observed. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Rhizobium meliloti nationallicence Population genetics nationallicence Polymerase chain reaction nationallicence IS fingerprinting nationallicence ERIC fingerprinting nationallicence Deliberate release nationallicence Persistence nationallicence Villadas Pablo J. Departamento de Microbiología, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain aut Burgos Pedro Departamento de Microbiología, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain aut Jording Doris Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany aut Selbitschka Werner Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany aut Pühler Alfred Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany aut Toro Nicolás Departamento de Microbiología, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 37-45 21:1<37 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00331.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00331.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Villadas Pablo J. Departamento de Microbiología, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain aut NATIONALLICENCE 700 1- Burgos Pedro Departamento de Microbiología, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain aut NATIONALLICENCE 700 1- Jording Doris Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany aut NATIONALLICENCE 700 1- Selbitschka Werner Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany aut NATIONALLICENCE 700 1- Pühler Alfred Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501 Bielefeld, Germany aut NATIONALLICENCE 700 1- Toro Nicolás Departamento de Microbiología, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 37-45 21:1<37 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford