caa a22 4500 397553072 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00333.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00333.x Extraction of methane-oxidizing bacteria from soil particles [Elektronische Daten] [Anders Priemé, J. Ileana Bonilla Sitaula, Åsa Kasimir Klemedtsson, Lars R. Bakken] We present a method for extraction of active methane (CH4)-oxidizing bacteria from soil samples. The method is based on physical dispersion of bacteria from the soil particles followed by separation of bacteria and soil particles by floatation in the density media Nycodenz or Percoll. Separation on Nycodenz produced very pure bacterial suspensions while separation on Percoll produced rather impure suspensions. However, more than 60% of the methane-oxidizing activity was irreversibly inhibited in the procedure using Nycodenz compared to less than 10% irreversible inhibition when Percoll was employed. The bacterial suspensions extracted from soil can be used to study the physiology and ecology of soil bacteria that oxidize methane at atmospheric concentrations. Our data indicated that these bacteria are extremely difficult to dislodge from particles compared to the majority of bacteria in soil. Tentatively, we interpret the strong attachment to long residence time (i.e. slow turnover) of the methane-oxidizing bacteria. A slow turnover/growth rate would explain why soil disturbances, like cultivation, have a long lasting effect on the oxidation of atmospheric methane in soil. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Methane oxidation nationallicence Bacterial adhesion nationallicence Extraction of soil bacteria nationallicence Nycodenz nationallicence Percoll nationallicence Priemé Anders Department of Population Biology, Copenhagen University, Universitetsparken 15, DK-2100 Copenhagen Ø, Denmark aut Bonilla Sitaula J. Ileana Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5040, N-1432 Aas, Norway aut Klemedtsson Åsa Kasimir Swedish Enviromental Research Institute, P.O. Box 47086, S-40258 Göteborg, Sweden aut Bakken Lars R. Department of Soil and Water Sciences, Agricultural University of Norway, P.O. Box 5028, N-1432 Aas, Norway aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 59-68 21:1<59 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00333.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00333.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Priemé Anders Department of Population Biology, Copenhagen University, Universitetsparken 15, DK-2100 Copenhagen Ø, Denmark aut NATIONALLICENCE 700 1- Bonilla Sitaula J. Ileana Department of Biotechnological Sciences, Agricultural University of Norway, P.O. Box 5040, N-1432 Aas, Norway aut NATIONALLICENCE 700 1- Klemedtsson Åsa Kasimir Swedish Enviromental Research Institute, P.O. Box 47086, S-40258 Göteborg, Sweden aut NATIONALLICENCE 700 1- Bakken Lars R. Department of Soil and Water Sciences, Agricultural University of Norway, P.O. Box 5028, N-1432 Aas, Norway aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 59-68 21:1<59 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford