caa a22 4500 397553080 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00332.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00332.x A systematic survey for thermophilic fermentative bacteria and archaea in high temperature petroleum reservoirs [Elektronische Daten] [Gino S. Grassia, Keith M. McLean, Philippé Glénat, John Bauld, Alan J. Sheehy] Production waters from 36 high temperature petroleum reservoirs were examined for the presence of thermophilic, fermentative microorganisms. The direct supplementation of production waters with glucose and either yeast extract, peptone, tryptone or casamino acid resulted in the isolation of thermophilic, fermentative microorganisms from 47% of the petroleum reservoirs examined. Three distinctive morphological groups were isolated from the production waters of petroleum reservoirs with depths ranging from 396-3048 metres, temperatures ranging from 21-130°C, salinities ranging from 2.8-128 gl−1 and pHs ranging from 6.0-8.5. Group 1 were pleomorphic rod-shaped bacteria, Group 2 were sheathed rod-shaped bacteria, and Group 3 were coccoid archaea. Partial characterisation of strains from one seawater-flooded petroleum reservoir and three non-waterflooded petroleum reservoirs tentatively identified some strains in Group 1 as members of the genera Thermoanaerobacter and Thermoanaerobacterium, Group 2 as members of the Thermotogales order, and Group 3 as members of the genus Thermococcus. Production water salinity determined the type of microorganisms that were isolated. Group 1 organisms were found primarily in petroleum reservoirs with salinities less than 30 g/l, while Group 2 and 3 organisms were found to dominate in more saline reservoirs. The successful isolation of thermophilic, fermentative microorganisms from petroleum reservoirs decreased significantly with increasing salinity and temperature. These findings support the existence of a deep biosphere where fermentative microorganisms are widespread. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Petroleum reservoir nationallicence Thermophilic fermenter nationallicence Thermoanaerobacter nationallicence Thermoanaerobacterium nationallicence Thermotogales nationallicence Thermococcus nationallicence Grassia Gino S. Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, ACT 2616, Australia aut McLean Keith M. CSIRO Division of Exploration Geoscience, Private Bag PO, Wembly, WA 6014, Australia aut Glénat Philippé Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, ACT 2616, Australia aut Bauld John Environmental Geoscience and Groundwater Division, Australian Geological Survey Organisation, GPO Box 378, Canberra, ACT 2601, Australia aut Sheehy Alan J. Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, ACT 2616, Australia aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 47-58 21:1<47 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00332.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00332.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grassia Gino S. Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, ACT 2616, Australia aut NATIONALLICENCE 700 1- McLean Keith M. CSIRO Division of Exploration Geoscience, Private Bag PO, Wembly, WA 6014, Australia aut NATIONALLICENCE 700 1- Glénat Philippé Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, ACT 2616, Australia aut NATIONALLICENCE 700 1- Bauld John Environmental Geoscience and Groundwater Division, Australian Geological Survey Organisation, GPO Box 378, Canberra, ACT 2601, Australia aut NATIONALLICENCE 700 1- Sheehy Alan J. Microbiology Research Unit, Faculty of Applied Science, University of Canberra, Belconnen, ACT 2616, Australia aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 47-58 21:1<47 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford