caa a22 4500 397553110 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00330.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00330.x Methyl fluoride, an inhibitor of methane oxidation and methane production [Elektronische Daten] [Peter Frenzel, Ulrike Bosse] Methyl fluoride (CH3F) has been reported to inhibit effectively aerobic CH4 oxidation, while not affecting methanogenesis. Currently it is applied in a variety of ecosystems, where oxidation and production of CH4 take place simultaneously. We tested the effects of CH3F on both processes in a flooded soil, in wetland plants (rice, Oryza sativa, and cottontail, Typha latifolia), and in a microbial mat. CH4 emission from rice microcosms increased after treatment with up to 1.9% CH3F, but decreased to less than the initial rate after 3.3% CH3F had been added. In anoxic incubations of rice roots and Typha we observed in vitro an instantaneous methanogenesis that in rice was inhibited by CH3F. Cottontail-associated methanogenesis was not affected by CH3F. In anoxic slurries of ricefield soil CH4 production was inhibited by CH3F. Even at concentrations as low as 1000 ppmv CH3F (≈ 40 μM) methanogenesis was reduced by about 75% as compared to the control without the inhibitor. Methanogenesis could be recovered partly when CH3F was flushed out with N2. In soil slurries with CH3F methanogenesis could be stimulated by addition of formate, but not by acetate. Acetate accumulated in soil slurries with CH3F to about the same amount as did CH4 in the control experiment without inhibitor. Methanogenesis in the hypersaline microbial mat is probably driven by methylated amines; it was not affected by CH3F. Hence, measurements of aerobic CH4 oxidation may be biased if acetoclastic methanogenesis plays a significant role, and if CH4 production and oxidation zones are closely coupled. This is to be expected especially in freshwater sediments, wetlands and ricefields. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Typha latifolia nationallicence Rice nationallicence Paddy soil nationallicence Methane production nationallicence Methane oxidation nationallicence Methyl fluoride nationallicence Inhibition nationallicence Acetate nationallicence Frenzel Peter Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut Bosse Ulrike Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 25-36 21:1<25 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00330.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00330.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frenzel Peter Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut NATIONALLICENCE 700 1- Bosse Ulrike Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/1(1996-09), 25-36 21:1<25 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford