caa a22 4500 397555180 CHVBK 20180308164759.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021251 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021251 Effects of Monophosphoryllipid-A on the Immunization of Mice with Keyhole Limpet Hemocyanin- and Muramyldipeptide-Ganglioside Gfpt1 Conjugates [Elektronische Daten] [Richard Jennemann, Bernhard L. Bauer, Renate Schmidt, Hans-Peter Elsässer, Herbert Wiegandt] Since it was considered that an active immunization against ganglioside Gfpt1 (IV2Fuc-, II3NeuAc-Gg4Cer) expressed by human small cell lung cancer cells may be beneficial in the treatment of this neoplasm in humans, an optimal mode of vaccination in model mice was investigated. A novel Gfpt1-muramyldipeptide conjugate (Gfpt1-MDP) was synthesized. Its ganglioside carbohydrate-directed immunogenicity in mice as measured by serum antibody titers was comparable to that of the previously described Gfpt1-keyhole limpet hemocyanin conjugate (Gfpt1-KLH). Similar immunogenicity was displayed by free Gfpt1 in muramyldipeptide-phosphoethanolamine-containing phosphatidyl-choline, -serine (PC, PS) liposomes. Immunization with Gfpt1-vaccines in the presence of monophosphoryllipid A (MPL), in general, raised titers of anti-Gfpt1 antibodies effectively. Immunization with PC, PS-liposomes containing unconjugated Gfpt1 and MPL stimulated the highest titers observed, thereby effectively preventing tumor growth in Balbc nu/nu-mice challenged with human small cell lung cancer cells. However, there was a strong crossreaction of these and most other sera with the structurally related and widely distributed ganglioside Gtet1 (II3NeuAc-Gg4Cer). Only immunization with Gfpt1-KLH conjugate in the presence of MPL stimulated selectively high anti-Gfpt1 antibody titers showing comparably low crossreactivity to ganglioside Gtet1. © by the Journal of Biochemistry Regular Papers nationallicence ganglioside IV2Fuc- nationallicence II3NeuAc-Gg4Cer nationallicence keyhole limpet hemocyanin nationallicence monophosphoryllipid-A nationallicence muramyldipeptide nationallicence small cell lung cancer nationallicence Jennemann Richard Zentrum für Operative Medizin II, Abtlg. Neurochirurgie, Philipps-Uniuersität Marburg Germany aut Bauer Bernhard L. Zentrum für Operative Medizin II, Abtlg. Neurochirurgie, Philipps-Uniuersität Marburg Germany aut Schmidt Renate Institut für Physiologische Chemie, Philipps-Uniuersität Marburg Germany aut Elsässer Hans-Peter Institut für Zytobiologie, Philipps-Uniuersität Marburg Germany aut Wiegandt Herbert Institut für Physiologische Chemie, Philipps-Uniuersität Marburg Germany aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 378-384 0021-924X 119:2<378 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021251 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021251 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jennemann Richard Zentrum für Operative Medizin II, Abtlg. Neurochirurgie, Philipps-Uniuersität Marburg Germany aut NATIONALLICENCE 700 1- Bauer Bernhard L. Zentrum für Operative Medizin II, Abtlg. Neurochirurgie, Philipps-Uniuersität Marburg Germany aut NATIONALLICENCE 700 1- Schmidt Renate Institut für Physiologische Chemie, Philipps-Uniuersität Marburg Germany aut NATIONALLICENCE 700 1- Elsässer Hans-Peter Institut für Zytobiologie, Philipps-Uniuersität Marburg Germany aut NATIONALLICENCE 700 1- Wiegandt Herbert Institut für Physiologische Chemie, Philipps-Uniuersität Marburg Germany aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 378-384 0021-924X 119:2<378 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford