caa a22 4500 397555199 CHVBK 20180308164759.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021225 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021225 Crystallization and Preliminary X-Ray Diffraction Study of Recombinant Human Eukaryotic Initiation Factor-4E [Elektronische Daten] [Shigenobu Morino, Koji Tomoo, Noriko Nishi, Koumei Okabe, Mitsunobu Doi, Toshimasa Ishida, Kunihiro Kitamura] Recombinant human eukaryotic initiation factor-4E (eIF-4E), purified by m7GTP-Sepha-rose 4B affinity chromatography, was used for crystallization. After concentration of the eIF-4E protein (7 mg/ml), the solution was subjected to crystallization by the hanging-drop method. Transparent needle crystals complexed with m7GTP were obtained from 50 mM 2-(Ar-morpholino)ethanesulfonic acid-KOH buffer (pH 6.5) containing 25% (w/v) polyethylene glycol 6000 and 0.2 M (NH4)2SO4. The crystals belong to tetragonal space group P, or P43, of Z=4, with unit-cell dimensions of a;=89.26, b = 89.26, and c=38.51 Å, and diffract beyond 2.1Å resolution. The Vm value was calculated to be 3.07 Å3/Da, which indicates a solvent content of 59.9%. © by the Journal of Biochemistry Rapid Communications nationallicence cap structure nationallicence complex nationallicence crystallization nationallicence eukaryotic initiation factor-4E nationallicence m7GTP nationallicence Morino Shigenobu Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut Tomoo Koji Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut Nishi Noriko Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut Okabe Koumei Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut Doi Mitsunobu Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut Ishida Toshimasa Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut Kitamura Kunihiro Research Center of Taisho Pharmaceutical Co., Ltd., 1-403 Yoshino-cho Ohmiya, Saitama 330 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 224-225 0021-924X 119:2<224 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021225 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021225 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morino Shigenobu Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut NATIONALLICENCE 700 1- Tomoo Koji Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut NATIONALLICENCE 700 1- Nishi Noriko Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut NATIONALLICENCE 700 1- Okabe Koumei Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut NATIONALLICENCE 700 1- Doi Mitsunobu Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut NATIONALLICENCE 700 1- Ishida Toshimasa Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences 2-10-65 Kawai, Matsubara, Osaka 580 aut NATIONALLICENCE 700 1- Kitamura Kunihiro Research Center of Taisho Pharmaceutical Co., Ltd., 1-403 Yoshino-cho Ohmiya, Saitama 330 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 224-225 0021-924X 119:2<224 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford