caa a22 4500 397555245 CHVBK 20180308164759.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021246 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021246 Expression of Carbohydrate-Binding Protein p33/41 in Human Tumor Cell Lines [Elektronische Daten] [Ayano Satoh, Eiji Takayama, Kyoko Kojima, Haruko Ogawa, Takao Yamori, Shigeo Sato, Tokuichi Kawaguchi, Takashi Tsuruo, Yoshimoto Katsura, Tatsuo Kina, Isamu Matsumoto] We previously reported a new type of lectin, p33/41 (annexin IV), which was isolated from a bovine tissue extract [Kojima, K.et al. (1992) J. Biol. Chem. 267, 20536-20539]. When the expression of p33/41 (annexin IV) was surveyed in the lysates of 39 human tumor cell lines by SDS-PAGE, followed by Western blot analysis with polyclonal anti-bovine p33/41 and monoclonal anti-annexin IV (Z016, Zymed) antibodies, 21 cell lines were found to be reactive with the polyclonal antibody, whereas all 39 cell lines were stained with Z016. These results together with those obtained with standard proteins, annexins IV and V, suggested that the monoclonal antibody, Z016, recognizes annexin V, but not p33/41 (annexin IV). Therefore, we performed cDNA cloning of human p33/41 (annexin IV) to prepare a recombinant protein and raised monoclonal antibodies against the protein. Northern blot analysis with the cDNA as a probe showed that a human colon cancer cell line, HT29, contains p33/41 (annexin IV) mRNA of two sizes, 2.0 and 3.0 kb. The two monoclonal antibodies, ASH and AS17, against the recombinant protein generated were useful for flow cytometric analysis, ELISA, Western blot analysis and immunoprecipitation. Flow cyto-metric analysis with AS17 showed that p33/41 (annexin IV) is located in the cytoplasm of HT29 cells, but not on the cell surface. However, one of the cell surface proteins first labeled with biotin and then solubilized with a detergent was immunoprecipitated with AS17. The results suggest the existence of a membrane spanning form of p33/41 (annexin TV). © by the Journal of Biochemistry Regular Papers nationallicence annexin nationallicence lectin nationallicence monoclonal antibody nationallicence tumor cell line nationallicence Satoh Ayano Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut Takayama Eiji Department of Molecular Pathology and Immunology, Chest Disease Research Institute, Kyoto University Shogoln-Kawaramachi 53, Sakyo-ku, Kyoto 606 aut Kojima Kyoko Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut Ogawa Haruko Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut Yamori Takao Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut Sato Shigeo Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut Kawaguchi Tokuichi Department of Pathology, Japanese Foundation for Cancer Research Kami-Ikebukuro, Toshima-ku, Tokyo 117 aut Tsuruo Takashi Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut Katsura Yoshimoto Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut Kina Tatsuo Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut Matsumoto Isamu Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 346-353 0021-924X 119:2<346 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021246 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021246 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Satoh Ayano Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Takayama Eiji Department of Molecular Pathology and Immunology, Chest Disease Research Institute, Kyoto University Shogoln-Kawaramachi 53, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Kojima Kyoko Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Ogawa Haruko Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 700 1- Yamori Takao Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut NATIONALLICENCE 700 1- Sato Shigeo Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut NATIONALLICENCE 700 1- Kawaguchi Tokuichi Department of Pathology, Japanese Foundation for Cancer Research Kami-Ikebukuro, Toshima-ku, Tokyo 117 aut NATIONALLICENCE 700 1- Tsuruo Takashi Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut NATIONALLICENCE 700 1- Katsura Yoshimoto Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut NATIONALLICENCE 700 1- Kina Tatsuo Cancer Chemotherapy Center, Kami-Ikebukuro Toshima-ku, Tokyo 117 aut NATIONALLICENCE 700 1- Matsumoto Isamu Department of Chemistry, Faculty of Science, Ochanomizu University 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 346-353 0021-924X 119:2<346 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford