caa a22 4500 397555296 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021245 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021245 Sex and Strain Differences in Constitutive Expression of Fatty Acid ω-Hydroxylase (CYP4A-Related Proteins) in Mice [Elektronische Daten] [Masahiro Hiratsuka, Tomomi Matsuura, Eriko Watanabe, Masuo Sato, Yasuo Suzuki] The constitutive expression of hepatic fatty acid hydroxylase was examined in both sexes of ddY mice by measuring the activities of lauric acid ω-hydroxylase (LAH). The activity of male mice was significantly higher than that of female mice. Such a sex difference of hepatic LAH activity was not observed in other strains of mice, including BALB/c and C57BL/6. To examine whether decreased total P450 activities caused low LAH activity levels in female ddY mice, ethoxycoumarin O-deethylase activity, which is exhibited by many P450s, was measured in both sexes of mice. This activity had no sex difference. The developmental regulation of hepatic fatty acid hydroxylase was then examined by making consecutive measurements of LAH activity in ddY mice. The activity is the same in immature male and female mice, but is differentiated in the sexually mature state. Furthermore, in male mice, orchiectomy caused a dramatic decrease in hepatic LAH activity and the activity was restored by testosterone treatment to the level of the intact animal. In female mice, ovariectomy and estradiol treatment had no effect on the activity, but testosterone treatment caused an increase in the activity. The above data are consistent with the constitutive expression of CYP4A-related proteins measured by using anti-rat CYP4A1 polyclonal antibody. Anti-CYP4A1 antibody inhibited LAH activity, but not lauric acid (ω-l)-hydroxylase activity. These results suggest that some factors associated with male sex hormone are involved in the regulation of hepatic fatty acid ω-hydroxylase in ddY mice. © by the Journal of Biochemistry Regular Papers nationallicence hormonal regulation nationallicence lauric acid ω-hydroxylase nationallicence mouse CYP4A nationallicence sex difference nationallicence strain difference nationallicence Hiratsuka Masahiro Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut Matsuura Tomomi Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut Watanabe Eriko Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut Sato Masuo Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut Suzuki Yasuo Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 340-345 0021-924X 119:2<340 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021245 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021245 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hiratsuka Masahiro Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut NATIONALLICENCE 700 1- Matsuura Tomomi Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut NATIONALLICENCE 700 1- Watanabe Eriko Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut NATIONALLICENCE 700 1- Sato Masuo Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut NATIONALLICENCE 700 1- Suzuki Yasuo Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-77 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 340-345 0021-924X 119:2<340 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford