caa a22 4500 397555326 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021233 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021233 Change in the Expression of C-Type Natriuretic Peptide and Its Receptor, B-Type Natriuretic Peptide Receptor, during Dedifferentiation of Chondrocytes into Fibroblast-Like Cells [Elektronische Daten] [Hiromi Hagiwara, Atsuto Inoue, Mayumi Furuya, Shoji Tanaka, Shigehisa Hirose] Chondrocytes derived from rat xiphoid cartilage dedifferentiated into fibroblast-like cells as the number of passages of the cells in culture increased. During in vitro dedifferentiation the growth of the cells was markedly suppressed. We had proposed previously that C-type natriuretic peptide (CNP) might be a potent antimitogenic factor for chondrocytes, and TGF-β1 induced a marked increase in CNP secretion of chondrocytes. Therefore, we investigated the expression of CNP, B-type natriuretic peptide receptor (NPR-B or GC-B), and TGF-β1 in this process. Radioimmunoassay and RNase protection analyses revealed passage-associated increase in CNP-like immunoreactivity and in levels of NPR-B mRNA, respectively. Northern blot analyses showed that the level of TGF-β1 mRNA decreased with increasing passage number. These results suggest that the expression of CNP and NPR-B might be involved in in vitro dedifferentiation of chondrocytes and TGF-β1 does not affect the increasing level of CNP during in vitro dedifferentiation. © by the Journal of Biochemistry Regular Papers nationallicence B-type natriuretic peptide receptor nationallicence chondrocyte nationallicence C-type natriuretic peptide nationallicence dedifferentiation nationallicence proliferation nationallicence Hagiwara Hiromi Research Center for Experimental Biology, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku, Yokohama 226 aut Inoue Atsuto Research Center for Experimental Biology, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku, Yokohama 226 aut Furuya Mayumi Suntory Institute for Biomedical Research Shimamoto, Mishima, Osaka 618 aut Tanaka Shoji Suntory Institute for Biomedical Research Shimamoto, Mishima, Osaka 618 aut Hirose Shigehisa Department of Biological Sciences, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku, Yokohama 226 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 264-267 0021-924X 119:2<264 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021233 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021233 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hagiwara Hiromi Research Center for Experimental Biology, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Inoue Atsuto Research Center for Experimental Biology, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Furuya Mayumi Suntory Institute for Biomedical Research Shimamoto, Mishima, Osaka 618 aut NATIONALLICENCE 700 1- Tanaka Shoji Suntory Institute for Biomedical Research Shimamoto, Mishima, Osaka 618 aut NATIONALLICENCE 700 1- Hirose Shigehisa Department of Biological Sciences, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku, Yokohama 226 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 264-267 0021-924X 119:2<264 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford