caa a22 4500 397555334 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021229 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021229 Age-Related Changes in Lipid Secretion of Perfused Livers from Male Wistar Rats Donors [Elektronische Daten] [Elena Bravo, Roberto Rivabene, Giovannella Bruscalupi, Annarica Calcabrini, Giuseppe Arancia, Alfredo Cantafora] Male Wistar rats show typical age-related variations in the distribution of high-density lipoprotein subfractions that include an increase in HDL1 and a decrease in HDL2 proportion. The role of liver in these variations was evaluated by studying the lipoprotein and bile secretions from perfused livers of 14±1 and 3.5±0.5 month old Wistar rats (adult and young animals, respectively). The lipid content of lipoproteins secreted from adult livers was higher in HDL2 fraction and lower in VLDL fraction. The lipid output did not show significant age-related variations in the case of HDL1 fraction. However, the lipoproteins secreted from adult livers contained a higher proportion of phospholipids, and a lower proportion of triacylglycerols in comparison with lipoproteins secreted by young livers. Therefore, the molar ratio of core to surface lipids was lower in lipoproteins secreted by adult livers. Adult livers showed a reduction in bile flow by about 37% with a significantly higher phospholipid secretion. These findings suggest that both the hepatic metabolism of glycerophospholipids and their repartition between plasma and bile compartments are affected by aging process. In conclusion, present data show that the age-related increase in plasma HDL1 proportion, previously observed in this rat strain in vivo, are not due to a higher liver secretion of these particles. Conversely, liver appears to have a major role in the age-related VLDL increase and in the variations of phospholipid lipoprotein secretion. © by the Journal of Biochemistry Regular Papers nationallicence aging nationallicence lipoprotein secretion nationallicence perfused liver nationallicence phospholipid nationallicence triacylglycerol nationallicence Bravo Elena Laboratories of Metabolism and Pathological Biochemistry and Wltrastructures, Istituto Superiore di Sanita' 00161, Rome aut Rivabene Roberto Ultrastructures, Istituto Superiore di Sanita' 00161, Rome aut Bruscalupi Giovannella Department of Cell and Developmental Biology, University La Sapienza 00161, Rome, Italy aut Calcabrini Annarica Ultrastructures, Istituto Superiore di Sanita' 00161, Rome aut Arancia Giuseppe Ultrastructures, Istituto Superiore di Sanita' 00161, Rome aut Cantafora Alfredo Laboratories of Metabolism and Pathological Biochemistry and Wltrastructures, Istituto Superiore di Sanita' 00161, Rome aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 240-245 0021-924X 119:2<240 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021229 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021229 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bravo Elena Laboratories of Metabolism and Pathological Biochemistry and Wltrastructures, Istituto Superiore di Sanita' 00161, Rome aut NATIONALLICENCE 700 1- Rivabene Roberto Ultrastructures, Istituto Superiore di Sanita' 00161, Rome aut NATIONALLICENCE 700 1- Bruscalupi Giovannella Department of Cell and Developmental Biology, University La Sapienza 00161, Rome, Italy aut NATIONALLICENCE 700 1- Calcabrini Annarica Ultrastructures, Istituto Superiore di Sanita' 00161, Rome aut NATIONALLICENCE 700 1- Arancia Giuseppe Ultrastructures, Istituto Superiore di Sanita' 00161, Rome aut NATIONALLICENCE 700 1- Cantafora Alfredo Laboratories of Metabolism and Pathological Biochemistry and Wltrastructures, Istituto Superiore di Sanita' 00161, Rome aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 240-245 0021-924X 119:2<240 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford