caa a22 4500 397555342 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021237 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021237 Change in the Expression of Hypopharyngeal-Gland Proteins of the Worker Honeybees (Apis melliferaL.) with Age and/or Role [Elektronische Daten] [Takeo Kubo, Masami Sasaki, Jun Nakamura, Hiromi Sasagawa, Kazuaki Ohashi, Hideaki Takeuchi, Shunji Natori] The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis using affinity-purified antibodies against three major proteins (50, 56, and 64 kDa) of the nurse-bee gland showed that they are synthesized selectively and secreted as bee-milk proteins. Immunofluorescence study showed that the proteins condense in the duct after secretion from acini. However, a major 70-kDa protein synthesized specifically in the forager-bee hypopharyngeal gland was identified as an α-glucosidase. Therefore, the hypopharyngeal gland seems to have two distinct states differentiated by synthesizing of different major proteins depending on the age-dependent role change. © by the Journal of Biochemistry Regular Papers nationallicence honeybee nationallicence hypopharyngeal gland nationallicence role change nationallicence royal jelly nationallicence α-glucosidase nationallicence Kubo Takeo Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut Sasaki Masami Faculty of Agriculture, Tamagawa University Machida, Tokyo 194 aut Nakamura Jun Faculty of Agriculture, Tamagawa University Machida, Tokyo 194 aut Sasagawa Hiromi Department of Insect Physiology and Behavior, National Institute of Sericultural and Entomological Science Tsukuba, Ibaraki 305 aut Ohashi Kazuaki Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut Takeuchi Hideaki Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut Natori Shunji Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 291-295 0021-924X 119:2<291 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021237 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021237 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubo Takeo Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Sasaki Masami Faculty of Agriculture, Tamagawa University Machida, Tokyo 194 aut NATIONALLICENCE 700 1- Nakamura Jun Faculty of Agriculture, Tamagawa University Machida, Tokyo 194 aut NATIONALLICENCE 700 1- Sasagawa Hiromi Department of Insect Physiology and Behavior, National Institute of Sericultural and Entomological Science Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Ohashi Kazuaki Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Takeuchi Hideaki Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Natori Shunji Faculty of Pharmaceutical Sciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 291-295 0021-924X 119:2<291 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford