caa a22 4500 397555350 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021243 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021243 Alterations in Activity of Protein Tyrosine Phosphatase SH-PTP1 in Autoimmune MRL/MpJ-lpr/lpr Mice [Elektronische Daten] [Akio Matsuda, Shu-ichi Matsuzawa, Koji Nakamura, Yusuke Mizuno, Kunimi Eikuchi] Activities of protein tyrosine kinase (PTK) and protein tyrosine phosphatase (PTP) in autoimmune MRL/MpJ-lpr/lpr mice (lpr mice) were measured and compared with the activities in the tissues from MRL/MpJ- +/+ mice (+/+ mice) as the control. In the spleen and liver, PTK activities in cytosol and membrane fractions were about 1.7- and 1.3-fold, respectively, higher in lpr mice than +/+ mice. PTP activities in cytosol and membrane fractions from lpr mice were 1.7- and 1.3-fold, respectively, higher in spleen, and 2.5- and 1.3-fold, respectively, higher in liver compared with those of the controls. These results demonstrate that the mutation of lpr gene resulted in elevation of PTK and PTP activities. Then, we measured the amounts and activities of SH-PTP1, a cytosolic PTP playing a crucial role in intracellular signaling from Fas antigen. The amounts of SH-PTP1 were about 4-fold larger in thymus, spleen, and lymphnodes than in liver, but there was no marked difference in the amounts between lpr and +/+ mice. On the other hand, activity of SH-PTP1 was definitely lower in lpr spleen and lymphnodes than +/+ spleen, but several times higher in lpr liver than +/+ liver. Tyrosine phosphorylation levels of SH-PTP1 in spleen of lpr and +/+ mice were similar. However, in liver, it was less phosphorylated in lpr than in +/+ mice. This hypophosphorylation might cause the activation of SH-PTP1 activity in lpr liver. © by the Journal of Biochemistry Regular Papers nationallicence autoimmune disease nationallicence lpr mice nationallicence protein tyrosine phosphatase nationallicence SH-PTP1 nationallicence tyrosine phosphorylation nationallicence Matsuda Akio Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut Matsuzawa Shu-ichi Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut Nakamura Koji Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut Mizuno Yusuke Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut Eikuchi Kunimi Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 329-333 0021-924X 119:2<329 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021243 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021243 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsuda Akio Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Matsuzawa Shu-ichi Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Nakamura Koji Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Mizuno Yusuke Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Eikuchi Kunimi Section of Biochemistry, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 329-333 0021-924X 119:2<329 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford