caa a22 4500 397555377 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021239 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021239 Molecular Cloning and Expression of a Mouse α-1,3 Fucosyltransferase Gene That Shows Homology with the Humanα-1,3 Fucosyltransferase IV Gene [Elektronische Daten] [Masayuki Ozawa, Takashi Muramatsu] The polymerase chain reaction was used to amplify and clone a fragment of a novel fucosyltransferase cDNA from mouse embryonal carcinoma F9 cells. Southern blot analysis of mouse genomic DNA with the cloned cDNA fragment revealed that there is a single copy of the fucosyltransferase gene per haploid genome. A 5 kb EcoRI restriction fragment of the genomic DNA hybridized with the cDNA has been cloned and a 1,697 bp fragment containing the sequence of the cDNA was sequenced. The nucleotide sequence and the deduced amino acid sequence of the clone showed a highest degree of homology with those of human α-1,3 fucosyltransferase IV, i.e. 77.1 and 75.5% identity, respectively. Consistent with the sequence similarity, the transfection of a 1,544 bp fragment under the control of the cytomegarovirus enhancer and β actin promoter into COS-1 cells confers α-1,3 fucosyltransferase activity on the cells, resulting in cell surface expression of SSEA-1. © by the Journal of Biochemistry Regular Papers nationallicence embryonal carcinoma cells nationallicence fucosyltransferase nationallicence gene nationallicence Lewis x antigen nationallicence SSEA-1 nationallicence Ozawa Masayuki Department of Biochemistry, Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima 890 aut Muramatsu Takashi Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumaicho, Showa-ku, Nagoya 466 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 302-308 0021-924X 119:2<302 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021239 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021239 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ozawa Masayuki Department of Biochemistry, Faculty of Medicine, Kagoshima University 8-35-1 Sakuragaoka, Kagoshima 890 aut NATIONALLICENCE 700 1- Muramatsu Takashi Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumaicho, Showa-ku, Nagoya 466 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 302-308 0021-924X 119:2<302 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford