caa a22 4500 397555385 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021241 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021241 Cytochrome P450rm from Rhodotorula minuta Catalyzes 4-Hydroxylation of Benzoate [Elektronische Daten] [Hideo Fukuda, Kiminori Nakamura, Eiki Sukita, Takahira Ogawa, Takao Fujii] Rhodotorula minuta, a red yeast, produces a cytochrome P450, tentatively named P450rm, catalyzing the formation of isobutene from isovalerate. We found that P450rm interacted with benzoate and the dissociation constant of P450rm for benzoate was 36μM. A reconstituted system that consisted of purified P450rm and cytochrome P450 reductase catalyzed the 4-hydroxylation of benzoate in addition to the formation of isobutene; the turnover rate was ∼40 nmol/min/nmol P450rm. The P450rm-monooxygenase system was specific for benzoate and did not catalyze hydroxylation of other aromatic carboxylates. Since only a benzoate 4-hydroxylase that requires tetrahydropteridine has been isolated to date, P460rm appears to be the first isolated cytochrome P450 that acts as a benzoate 4-hydroxy-lase. The P450rm-monooxygenase system in microsomes of R. minuta might function in the degradation of L-phenylalanine on the pathway to β-ketoadipate. © by the Journal of Biochemistry Regular Papers nationallicence benzoate 4-hydroxylase nationallicence cytochrome P450 nationallicence formation of isobutene nationallicence P460rm nationallicence Rhodotorula minuta nationallicence Fukuda Hideo Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut Nakamura Kiminori Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut Sukita Eiki Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut Ogawa Takahira Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut Fujii Takao Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 314-318 0021-924X 119:2<314 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021241 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021241 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukuda Hideo Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut NATIONALLICENCE 700 1- Nakamura Kiminori Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut NATIONALLICENCE 700 1- Sukita Eiki Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut NATIONALLICENCE 700 1- Ogawa Takahira Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut NATIONALLICENCE 700 1- Fujii Takao Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 314-318 0021-924X 119:2<314 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford