caa a22 4500 397555423 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199602 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021227 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021227 Structure of Mouse Interferon Stimulated Gene Factor 3γ(ISGF3γ/ p48) cDNA and Chromosomal Localization of the Gene [Elektronische Daten] [Wakako Suhara, Mitsutoshi Yoneyama, Hiromichi Yonekawa, Takashi Fujita] Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-α and β (type I EFNs). Upon stimulation, the regulatory subunits, p84/91 and pll3, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA binding subunit, ISGF3γ, into the active ISGF3 in the nucleus. Thus, ISGF3 plays a primary role in the transmission of a signal from the cell surface to the nucleus. In this report, we describe the cloning of a mouse cDNA encoding a polypeptide homologous to human ISGF3γ. Comparison of the deduced amino acid sequences revealed the middle region was significantly different between mouse and man. The mouse cDNA was shown to encode a functional ISGF3 subunit by means of an in vitro constitution assay. Furthermore, the locus of the ISGF3γ gene, designated as Isgf3g, was mapped to distal mouse chromosome 14 by linkage analysis using an intersubspecific backcross typing panel. © by the Journal of Biochemistry Rapid Communications nationallicence amino acid sequence nationallicence chromosomal localization nationallicence interferon nationallicence interferon stimulated gene factor 3 nationallicence reconstitution nationallicence Suhara Wakako Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Yoneyama Mitsutoshi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Yonekawa Hiromichi Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Fujita Takashi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 119/2(1996-02), 231-234 0021-924X 119:2<231 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021227 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021227 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suhara Wakako Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Yoneyama Mitsutoshi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Yonekawa Hiromichi Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Fujita Takashi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18+22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/2(1996-02), 231-234 0021-924X 119:2<231 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford