caa a22 4500 397555431 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021522 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021522 Automated Chemical Synthesis of Biologically Active tRNA Having a Sequence Corresponding to Ascaris suum Mitochondrial tRNAMet toward NMR Measurements [Elektronische Daten] [Takashi Ohtsuki, Ravi Vinayak, Yoh-ichi Watanabe, Kiyoshi Kita, Gota Kawai, Kimitsuna Watanabe] RNA samples corresponding to Ascaris suum mitochondrial tRNAMet were chemically and automatically synthesized in amounts sufficient for NMR measurement. Conventional and rapid deprotection methods gave tRNA samples with the same amino acid-accepting activity as those prepared by other method; enzymatic synthesis, and enzymatic ligation of chemically synthesized fragments. The synthetic tRNA showed the same 1H-NMR spectrum in the iminoproton region as the ligated tRNA. This rapid and reliable preparation method thus provides biologically active tRNA for NMR measurement, and further, it is applicable for synthesis of other large synthetic RNAs, by combining the site-specific isotopic labeling method. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence Ascarts suum nationallicence chemical synthesis nationallicence mitochondria nationallicence NMR nationallicence tRNA nationallicence Ohtsuki Takashi Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut Vinayak Ravi Applied Biosystems, Perkin Elmer Corporation, 850 Lincoln Center Drive Foster City CA 94404, USA aut Watanabe Yoh-ichi Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut Kita Kiyoshi Department of Parasitology, Institute of Medical Science, The University of Tokyo Shiroganedai, Minato-ku, Tokyo 108 aut Kawai Gota Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut Watanabe Kimitsuna Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1070-1073 0021-924X 120:6<1070 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021522 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021522 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohtsuki Takashi Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut NATIONALLICENCE 700 1- Vinayak Ravi Applied Biosystems, Perkin Elmer Corporation, 850 Lincoln Center Drive Foster City CA 94404, USA aut NATIONALLICENCE 700 1- Watanabe Yoh-ichi Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut NATIONALLICENCE 700 1- Kita Kiyoshi Department of Parasitology, Institute of Medical Science, The University of Tokyo Shiroganedai, Minato-ku, Tokyo 108 aut NATIONALLICENCE 700 1- Kawai Gota Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut NATIONALLICENCE 700 1- Watanabe Kimitsuna Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1070-1073 0021-924X 120:6<1070 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford