caa a22 4500 39755544X CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021540 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021540 Role of Calcium in Tumor Necrosis Factor-α Production by Activated Macrophages [Elektronische Daten] [Naoko Watanabe, Junko Suzuki, Yoshiro Kobayashi] The role of calcium in the production of tumor necrosis factor-α (TNF-α) by the lipopolysaccharide (LPS)-stimulated macrophage cell line, J774.1, was investigated. Flow cytometric measurement of intracellular calcium concentration ( [Ca]1)using 2-(3,6-bis(acetyl-oxy)-2,7-dichloro-9H-xanthen-9-yl)benzoic acid (fluo-3)-loaded J774.1 cells revealed that LPS at more than 0.1 μg/ml increased [Ca]1 transiently in the presence or absence of serum, and that a mixture of a calcium chelator, ethyleneglycol-bis(β-aminoethyl ether) N,N,N',N' -tetraacetic acid (EGTA), and a calcium release blocker from intracellular store sites, 8-(diethyiamino)-octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8), inhibited the [Ca]1 response induced by LPS. In concordance with this, production of TNF-α was inhibited by EGTA and/or TMB-8. These reagents also reduced the level of TNF-α mRNA significantly. These results indicate that the transient increase of [Ca]1 plays a role in LPS-induced expression of TNF-α by the macrophage cell line. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence calcium nationallicence calcium chelator nationallicence lipopolysacchride (LPS) nationallicence tumor necrosis factor-α(TNF-α) nationallicence Watanabe Naoko Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274 aut Suzuki Junko Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274 aut Kobayashi Yoshiro Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1190-1195 0021-924X 120:6<1190 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021540 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021540 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watanabe Naoko Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274 aut NATIONALLICENCE 700 1- Suzuki Junko Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274 aut NATIONALLICENCE 700 1- Kobayashi Yoshiro Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1190-1195 0021-924X 120:6<1190 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford