caa a22 4500 397555482 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021527 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021527 Complete Nucleotide Sequences of the Genes Encoding Translation Elongation Factors 1α and 2 from a microsporidian parasite, Glugea plecoglossi: Implications for the Deepest Branching of Eukaryotes [Elektronische Daten] [Takashi Kamaishi, Tetsuo Hashimoto, Yoshihiro Nakamura, Yakuta Masuda, Fuminori Nakamura, Ken-ichi Okamoto, Makoto Shimizu, Masami Hasegawa] Complete nucleotide sequences of the genes putatively encoding translation elongation factors 1α (EF-la) and 2 (EF-2) from a mitochondrion-lacking protozoan, Glugea pieco giossi, that belongs to microsporidians were determined. The deduced amino acid sequeneces of the putative EF-lα and EF-2 of Gl. plecoglossi showed very unusual features compared with typical eukaryotic sequences. The degree of divergence was especially great in the EF-lα sequence, although it clearly showed a eukaryotic feature when aligned with homologs from the three primary kingdoms. Phylogenetic analyses of EF-lα and EF-2 on the basis of the maximum likelihood method of protein phylogeny clearly and consistently suggested that among eukaryotic species being analyzed, Gl. plecoglossi and another mitochondrion-lacking protozoan, Giardia lamblia, respectively represent the earliest and the second earliest offshoots of eukaryotes. When the EF-α and EF-2 phylogenies were totally evaluated, the earliest divergence of Gl. plecoglossi in eukaryotes became more clearly confirmed. If the phylogenetic relationships inferred from the present analysis are correct, microsporidians might be extremely ancient eukaryotes that diverged before the occurrence of mitochondrial symbiosis. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence ancient divergence of eukaryotes nationallicence elongation factor nationallicence Glugea plecoglossi nationallicence microsporidians nationallicence mitochondrion-lacking protozoa nationallicence Kamaishi Takashi Department of Medical Biology, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut Hashimoto Tetsuo The Institute of Statistical Mathematics 4-6-7 Minarni-Aanbu, Minato-ku, Tokyo 106 aut Nakamura Yoshihiro Laboratory of Gene Manipulation 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut Masuda Yakuta Department of Biological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut Nakamura Fuminori Department of Medical Biology, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut Okamoto Ken-ichi Department of Medical Biology, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut Shimizu Makoto Department of Fisheries, Faculty of Agriculture, The University of Tokyo 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113 aut Hasegawa Masami The Institute of Statistical Mathematics 4-6-7 Minarni-Aanbu, Minato-ku, Tokyo 106 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1095-1103 0021-924X 120:6<1095 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021527 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021527 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamaishi Takashi Department of Medical Biology, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut NATIONALLICENCE 700 1- Hashimoto Tetsuo The Institute of Statistical Mathematics 4-6-7 Minarni-Aanbu, Minato-ku, Tokyo 106 aut NATIONALLICENCE 700 1- Nakamura Yoshihiro Laboratory of Gene Manipulation 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut NATIONALLICENCE 700 1- Masuda Yakuta Department of Biological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut NATIONALLICENCE 700 1- Nakamura Fuminori Department of Medical Biology, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut NATIONALLICENCE 700 1- Okamoto Ken-ichi Department of Medical Biology, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku Tokyo 142 aut NATIONALLICENCE 700 1- Shimizu Makoto Department of Fisheries, Faculty of Agriculture, The University of Tokyo 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Hasegawa Masami The Institute of Statistical Mathematics 4-6-7 Minarni-Aanbu, Minato-ku, Tokyo 106 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1095-1103 0021-924X 120:6<1095 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford