caa a22 4500 397555490 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021537 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021537 Cloning and Sequencing of cDNAs Encoding Plasma α-Macroglobulin and Murinoglobulin from Guinea Pig: Implications for Molecular Evolution of α-Macroglobulin Family [Elektronische Daten] [Hiromitu Iwasaki, Yasuyuki Suzuki, Hyogo Sinohara] Several clones encoding plasma α-macroglobulin and murinoglobulin were isolated from guinea pig liver cDNA library and sequenced. The clones for α-macroglobulin contained overlapping sequences which together spanned a stretch of 4,546 nucleotides with one open reading frame coding for 1,476 amino acid residues. The clones for murinoglobulin contained overlapping sequences which together spanned a stretch of 4,578 nucleotides with one open reading frame coding for 1,464 amino acid residues. The phylogenetic analyses of 11 proteins of the α-macroglobulin family revealed that the mammalian tetrameric α-macroglobulins consist of two main branches: αM-1 subfamily (rat α1- and mouse α-macroglobulins) and αM-2 subfamily (human α2-, rat α2-, and guinea pig α-macroglobulins). This dichotomy is in good accordance with their immunological, chemical, and physicochemical properties, and indicates that guinea pig α-macroglobulin is orthologous to human and rat α2-macroglobulins but paralogous to rat α1- and mouse α-macroglobulins. The divergence of the two subfamilies was a phylogenetically ancient event which occurred around the separation of metatherians and eutherians. The genes of the two subfamilies have been maintained in the rat, but either one became extinct in the mouse, guinea pig, or human. The tree also shows that guinea pig murinoglobulin forms one clade with mouse and rat murinoglobulins (α1-inhibitor3 prior to joining the 7αM-2 lineage, and suggests that murinoglobulin is not a primitive form of tetrameric α-macroglobulin, but rather has evolved under selective pressure which is different from that of the tetrameric paralogues. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence guinea pig α-macroglobulin nationallicence molecular evolution nationallicence murinoglobulin nationallicence rodent phylogeny nationallicence Iwasaki Hiromitu Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka 589 aut Suzuki Yasuyuki Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka 589 aut Sinohara Hyogo Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka 589 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1167-1175 0021-924X 120:6<1167 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021537 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021537 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwasaki Hiromitu Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka 589 aut NATIONALLICENCE 700 1- Suzuki Yasuyuki Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka 589 aut NATIONALLICENCE 700 1- Sinohara Hyogo Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka 589 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1167-1175 0021-924X 120:6<1167 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford