caa a22 4500 397555539 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021521 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021521 Dinuclear Lanthanum(III) Complex for Efficient Hydrolysis of RNA [Elektronische Daten] [Morio Yashiro, Akira Ishikubo, Makoto Komiyama] A dinuclear La3+ complex hydrolyzes the phosphodiester bond in diribonucleotides efficiently under mild conditions. The rate of hydrolysis is remarkably accelerated on the complex formation between and TPHP (TPHP=N,N,N',N'-tetrakis [(2-pyridyl)meth-yl]-2-hydroxy-1,3-dianinopropane), and the highest activity is attained when the dinu-clear structure is formed. The half-life of ApA hydrolysis by the dinuclear La3+ complex (1 mM) is 350 s, whereas that by the free La3+ ion (1 mM) is 23,000 s at pH 7.2 and 50°C. The dinuclear La3+ complex is promising as a catalytic center of artificial nucleases. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence artificial metalloenzyme nationallicence dinuclear metal complex nationallicence hydrolysis nationallicence ribonucleotide dimer nationallicence Yashiro Morio Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 aut Ishikubo Akira Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 aut Komiyama Makoto Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1067-1069 0021-924X 120:6<1067 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021521 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021521 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yashiro Morio Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Ishikubo Akira Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Komiyama Makoto Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1067-1069 0021-924X 120:6<1067 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford