caa a22 4500 397555547 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021526 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021526 Biosynthetic Transport of a Major Lysosome-Associated Membrane Glycoprotein 2, Lamp-2: A Significant Fraction of Newly Synthesized Lamp-2 Is Delivered to Lysosomes by Way of Early Endosomes [Elektronische Daten] [Kenji Akasaki, Akihiro Michihara, Yoshiteru Fujiwara, Kouichi Mibuka, Hiroshi Tsuji] Lysosomal membranes contain two highly glycosylated proteins, designated as lamp-1 and lamp-2, as major components. Lamp-1 and lamp-2 are similar to each other in the protein structure. Here, we investigated the biosynthetic transport of lamp-2 through the endocytic vacuoles in cultured rat hepatocytes in comparison with that of lamp-1, which has previously been studied [Akasaki et at. (1995) Exp. Cell Res. 220, 464-473]. Newly synthesized lamp-2 (NS-lamp-2) was transported to the trans-Golgi from rough endoplasmic reticulum with a half time (t1/2) of 32 min, more slowly than NS-lamp-1 (t1/2=13 min) After leaving the trans-Golgi, NS-lamp-2 is transferred to at least three compartments; the cell surface (t1/2=47 min), cell peripheral early endosomes (t1/2=38 min) and perinuclear late endosomes (t1/2 mm). NS-lamp-2 transported to any compartment is delivered finally to lysosomes (t1/2=90 min). A significant fraction of NS-lamp-1 (45% of the total) was transported from the trans-Golgi to early endosomes, and then delivered to dense lyso somes via perinuclear late endosomes, whereas a major portion of NS-lamp-1 follows an intracellular route to late endosomes without passing through the cell periphery. NS-lamp 2 leaves the cell peripheral region more rapidly than NS-lamp-1. The kinetic and quantita-tive data for biosynthetic transport of NS-lamp-2 to early endosomes and the cell surface indicate that NS-lamp-2 may be transported first to early endosomes, from which a small portion of it (˜3.5% of the total) moves to the plasma membrane via a recycling system. In contrast, a small fraction of NS-lamp-1 is transported to the plasma membrane directly from the trans-Golgi, since NS-lamp-1 is delivered to the plasma membrane and early endosomes with almost the same half times. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence biosynthesis nationallicence endocytosis nationallicence endosome nationallicence lysosome nationallicence membrane nationallicence glycoprotein nationallicence Akasaki Kenji Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut Michihara Akihiro Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut Fujiwara Yoshiteru Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut Mibuka Kouichi Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut Tsuji Hiroshi Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1088-1094 0021-924X 120:6<1088 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021526 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021526 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akasaki Kenji Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut NATIONALLICENCE 700 1- Michihara Akihiro Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut NATIONALLICENCE 700 1- Fujiwara Yoshiteru Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut NATIONALLICENCE 700 1- Mibuka Kouichi Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut NATIONALLICENCE 700 1- Tsuji Hiroshi Faculty of Pharmacy and Pharmaceutical Sciences, Gakuencho, Fukuywna University, Fukuyama, Hiroshima 729-02 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1088-1094 0021-924X 120:6<1088 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford