caa a22 4500 397555555 CHVBK 20180308164800.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021538 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021538 Comparison of ncd and Kinesin Motor Domains by Circular Dichroism Spectroscopy [Elektronische Daten] [Takashi Shimizu, Hisayuki Morii] ncd is a microtubule motor protein from Drosophila, having a 40 kDa domain homologous to the kinesin motor domain. In the present study, we investigated the circular dichroism (CD) spectra of the ncd motor domain in comparison with those of the kinesin motor domain. Although the two are about 40% identical in amino acid sequence, and recent X-ray crystallographic studies [Sablin, Kull, Cooke, Vale, and Fletterick (1996) Nature 380,555-559; Kull, Sablin, Lau, Fletterick, and Vale (1996) Nature 380, 550-555] indicate that their core structures are nearly identical, the far UV CD spectra of ncd and kinesin motor domains, both being monomeric, were considerably different from each other, suggesting a significant difference in the secondary, especially loop structures. The motor domain of ncd, like that of kinesin, contains tightly associating APP even after purification. We removed ADP from the ncd motor domain by gel filtration in the presence of EDTA and high salt. The resultant protein, however, was likely to be in an inactive state, since it bound ATP slowly. The far UV CD spectrum of the ncd motor domain devoid of ADP was nearly identical to that of the ncd motor domain with bound ADP. This indicated that the removal of ADP did not affect the backbone structure in the presence of high salt. On the other hand, the near UV CD spectrum of the ADP-free ncd motor domain differed from that of the ncd motor domain. ADP complex, one possibility being that the local conformation was changed upon removal of bound ADP. The near UV CD spectra of kinesin motor domain also showed a difference between the ADP-bound form and the nucleotide-free form, although the difference was much smaller. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence CD nationallicence kinesin nationallicence molecular motor nationallicence ncd nationallicence Shimizu Takashi National Institute of Bioscience and Human-Technology Tsukuba, Ibaraqi 305 aut Morii Hisayuki National Institute of Bioscience and Human-Technology Tsukuba, Ibaraqi 305 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1176-1181 0021-924X 120:6<1176 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021538 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021538 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimizu Takashi National Institute of Bioscience and Human-Technology Tsukuba, Ibaraqi 305 aut NATIONALLICENCE 700 1- Morii Hisayuki National Institute of Bioscience and Human-Technology Tsukuba, Ibaraqi 305 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1176-1181 0021-924X 120:6<1176 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford