caa a22 4500 397555636 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021541 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021541 Differential Expression of Laminin-5/Ladsin Subunits in Human Tissues and Cancer Cell Lines and Their Induction by Tumor Promoter and Growth Factors [Elektronische Daten] [Hiroto Mizushima, Yohei Miyagi, Yamato Kikkawa, Naoki Yamanaka, Hidetaro Yasumitsu, Kazuaki Misugi, Kaoru Miyazaki] We previously reported a new laminin variant containing laminin γ2 (or B2t) chain, ladsin, which exerted prominent cell-scattering, cell-adhesion, and cell-migration activities. In the present study, this laminin was further characterized, and gene expression of its three subunits in various human tissues and cancer cell lines was examined by Northern blotting. cDNA cloning of the largest subunit of ladsin and partial amino acid sequencing of its β (or B1) subunit revealed that ladsin was identical to laminin-5 (kalinin/epiligrin/nicein). Among various human tissues, placenta, lung, and fetal kidney expressed high levels of mRNAs for the three subunits of laminin-5 (laminin α3EPA, β3 and γ2 chains). Most gastric and squamous carcinoma cell lines constitutively expressed all of the three subunit mRNAs, while other types of carcinoma cell lines expressed one or two of them. The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF) strongly enhanced the gene expression of the three subunits, increasing 2 to 8- fold the secretion of laminin-5 from carcinoma cells into culture medium. However, TPA treatment did not increase the secretion of laminin β1 chain, a subunit of laminins-1, −3, and −6. The unique properties and inducibility by TPA and EGF of laminin-5 suggest that it is associated with growth and migration of cancer cells. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence cell adhesion nationallicence epidermal growth factor nationallicence gastric cancer nationallicence laminin-5 nationallicence tumor promoter nationallicence Mizushima Hiroto Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut Miyagi Yohei Department Second Pathology, Yokohama City University School of Medicine 3-9 Fukuura, Kanazawa-ku, Yokohama 236 aut Kikkawa Yamato Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut Yamanaka Naoki Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut Yasumitsu Hidetaro Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut Misugi Kazuaki Department Second Pathology, Yokohama City University School of Medicine 3-9 Fukuura, Kanazawa-ku, Yokohama 236 aut Miyazaki Kaoru Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1196-1202 0021-924X 120:6<1196 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021541 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021541 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizushima Hiroto Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut NATIONALLICENCE 700 1- Miyagi Yohei Department Second Pathology, Yokohama City University School of Medicine 3-9 Fukuura, Kanazawa-ku, Yokohama 236 aut NATIONALLICENCE 700 1- Kikkawa Yamato Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut NATIONALLICENCE 700 1- Yamanaka Naoki Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut NATIONALLICENCE 700 1- Yasumitsu Hidetaro Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut NATIONALLICENCE 700 1- Misugi Kazuaki Department Second Pathology, Yokohama City University School of Medicine 3-9 Fukuura, Kanazawa-ku, Yokohama 236 aut NATIONALLICENCE 700 1- Miyazaki Kaoru Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University 641-12 Maioka-cho, Totsuka-ku, Yokohama 244 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1196-1202 0021-924X 120:6<1196 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford