caa a22 4500 397555652 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021533 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021533 Effects of Methionine and Cu2+ on the Expression of Tyrosinase Activity in Streptomyces castaneoglobisporus [Elektronische Daten] [Kayo Ikeda, Tsutomu Masujima, Masanori Sugiyama] Streptomyces castaneoglobisporus HUT6202 expresses an enzyme, tyrosinase, responsible for the production of melanin-like pigments. The present study revealed that the tyrosinase synthesis by the microorganism was induced about 80-fold, when young cells cultured for 6h were incubated with methionine (Met) to the mid-log phase of growth, in comparison to without this amino acid. The Met-induced tyrosinase synthesis was inhibited by the addition of rifampicin and chloramphenicol, suggesting that transcriptional and translational events are necessary for the induction. We found that the addition of Cu2+ to the culture medium brings forward the period of expression of Met-induced tyrosinase activity. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Cu2+-carrier protien nationallicence induction by methionine nationallicence melanin pigment nationallicence S. castaneoglobisporus nationallicence trysonase from Streptomyces nationallicence Ikeda Kayo Institute of Pharmaceutical Sciences, Hiroshima University School of Medicme, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Masujima Tsutomu Institute of Pharmaceutical Sciences, Hiroshima University School of Medicme, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut Sugiyama Masanori Institute of Pharmaceutical Sciences, Hiroshima University School of Medicme, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1141-1145 0021-924X 120:6<1141 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021533 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021533 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ikeda Kayo Institute of Pharmaceutical Sciences, Hiroshima University School of Medicme, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Masujima Tsutomu Institute of Pharmaceutical Sciences, Hiroshima University School of Medicme, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 700 1- Sugiyama Masanori Institute of Pharmaceutical Sciences, Hiroshima University School of Medicme, Kasumi 1-2-3, Minami-ku, Hiroshima 734 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1141-1145 0021-924X 120:6<1141 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford