caa a22 4500 397555660 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021523 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021523 Molecular Cloning and Characterization of a Novel Isoform of the Human UDP-Galactose Transporter, and of Related Complementary DNAs Belonging to the Nucleotide-Sugar Transporter Gene Family [Elektronische Daten] [Nobuhiro Ishida, Nobuhiko Miura, Shigemi Yoshioka, Masao Kawakita] We described recently the molecular cloning of human UDP-galactose transporter 1 (hUGT1) [Miura, N. et al. (1998) J. Biochem. 120, 238-241]. Now we have characterized its isoform, hUGT2, that is most likely generated through the alternative splicing of a transcript derived from the UGT genomic gene, that also codes for hUGT1. Introduction of the open reading frame sequence of hUGT2 into a mouse cell line, Had-1, that lacks the UDP-galactose transporter, complemented the genetic defect of the mutant, as judged from the lectin-sensitivity spectra of the transformants and the nucleotide-sugar transporting activity of microsomal vesicles isolated from them. UGT-related genes were found through a BLAST search of dbEST based on their significant similarity with hUGT genes. We report here cDNA clones belonging to two subfamillies of the nucleotide-sugar transporter gene family. One is the human CMP-sialic acid transporter gene, and the other is a group of homologous genes with an undefined function that are distributed in man, mouse, and rat, and show significant similarity to the yeast UDP-N-acetylglucosainine transporter. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence CMP-sialic acid transporter nationallicence nucleotide-sugar transporter nationallicence UDP)I-galactose transporter nationallicence UDP-N-acetylglucosamine transporter nationallicence Ishida Nobuhiro Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut Miura Nobuhiko Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut Yoshioka Shigemi Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut Kawakita Masao Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1074-1078 0021-924X 120:6<1074 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021523 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021523 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishida Nobuhiro Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Miura Nobuhiko Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Yoshioka Shigemi Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Kawakita Masao Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/6(1996-12), 1074-1078 0021-924X 120:6<1074 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford