caa a22 4500 397555741 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021196 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021196 Expression of β3-Adrenoceptor and Stimulation of Glucose Transport by β3-Agonists in Brown Adipocyte Primary Culture [Elektronische Daten] [Hideki Nikami, Yasutake Shimizu, Michihiro Sumida, Yasuhiko Minokoshi, Toshihide Yoshida, Masayuki Saito, Takashi Shimazu] Precursor cells of brown adipocytes were isolated from the interscapular brown fat of newborn rats and cultured on collagen-coated dishes. When confluent cells were treated with dexamethasone, mRNAs for muscle/adipocyte type of glucose transporter, hormone-sensitive lipase, and CCAAT/enhancer binding protein α were increased remarkably, confirming a predominant effect of dexamethasone on the terminal differentiation of the cultured cells. Effects of dexamethasone on the expression of three subtypes of β-adrenoceptor were also examined. β1- and β2-adrenoceptor mRNAs remained constant regardless of dexamethasone-treatment, while β3-adrenoceptor mRNA was present only in dexamethasone-treated differentiated cells. To assess the metabolic response mediated by β3-adrenoceptor, glucose transport into the cells was estimated. Norepinephrine enhanced glucose transport in dexamethasone-treated differentiated cells, but not in undifferentiated cells. β3-Adrenergic agonists mimicked completely the stimulatory effect of norepinephrine at concentrations lower by two orders of magnitude. These results suggest that the β3-adrenoceptor is expressed during the course of differentiation in brown adipocytes and plays a significant role in the response of glucose transport to adrenergic stimulation. © by the Journal of Biochemistry Regular Papers nationallicence β9-adrenoceptor nationallicence β3-agonist nationallicence brown adipocyte nationallicence glucose transport nationallicence norepinephrine nationallicence Nikami Hideki Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University Kita-ku, Sapporo 060 aut Shimizu Yasutake Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut Sumida Michihiro Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut Minokoshi Yasuhiko Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut Yoshida Toshihide First Department of Internal Medicine, Kyoto Prefectural University of Medicine Sakyo-ku, Kyoto 602 aut Saito Masayuki Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University Kita-ku, Sapporo 060 aut Shimazu Takashi Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 120-125 0021-924X 119:1<120 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021196 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021196 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nikami Hideki Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Shimizu Yasutake Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut NATIONALLICENCE 700 1- Sumida Michihiro Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut NATIONALLICENCE 700 1- Minokoshi Yasuhiko Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut NATIONALLICENCE 700 1- Yoshida Toshihide First Department of Internal Medicine, Kyoto Prefectural University of Medicine Sakyo-ku, Kyoto 602 aut NATIONALLICENCE 700 1- Saito Masayuki Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Shimazu Takashi Department of Medical Biochemistry, Ehime University School of Medicine Shigenobu, Ehime 791-02 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 120-125 0021-924X 119:1<120 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford