caa a22 4500 39755575X CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021218 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021218 Phylogenetic Position of Kinetoplastid Protozoa Inferred from the Protein Phylogenies of Elongation Factors 1α and 2 [Elektronische Daten] [Yoshihiro Nakamura, Tetsuo Hashimoto, Takashi Kamaishi, Jun Adachi, Fuminori Nakamura, Ken-ichi Okamoto, Masami Hasegawa] Partial regions of the mRNA encoding a major part of translation elongation factor 2 (EF-2) from a kinetoplastid protozoan, Trypanosoma cruzi, were amplified by means of polymerase chain reaction and their primary structures were analyzed. The deduced amino acid sequence was aligned with those of other eukaryotic and archaebacterial EF-2s, and the phylogenetic relationships among eukaryotes were inferred by the maximum likelihood (ML) method. ML analyses of EF-2 phylogeny using six different stochastic models of amino acid substitutions consistently suggested that the phylogenetic position of T. cruzi is likely to be closer to higher eukaryotes than that inferred from the phylogeny of small subunit ribosomal RNA (SrRNA). These results are consistent with those for the elongation factor lα (EF-lα) phylogeny. When the EF-lα and EF-2 phylogenies were totally evaluated, it became much clearer that the divergence of T. cruzi occurred later than that of a mitochondrion-lacking protozoan, Entamoeba histolytica, although this is not conclusive. © by the Journal of Biochemistry Regular Papers nationallicence early evolution of eukaryotes nationallicence elongation factor nationallicence kinetoplastid protozoa nationallicence protein phylogeny nationallicence Trypanosoma cruzi nationallicence Nakamura Yoshihiro Laboratory of Gene Manipulation, School of Medicine, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Hashimoto Tetsuo Department of Medical Biology, School of Medicine, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Kamaishi Takashi The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut Adachi Jun Department of Medical Biology, School of Medicine, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Nakamura Fuminori The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut Okamoto Ken-ichi The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut Hasegawa Masami The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 70-79 0021-924X 119:1<70 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021218 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021218 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Yoshihiro Laboratory of Gene Manipulation, School of Medicine, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Hashimoto Tetsuo Department of Medical Biology, School of Medicine, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Kamaishi Takashi The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut NATIONALLICENCE 700 1- Adachi Jun Department of Medical Biology, School of Medicine, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Nakamura Fuminori The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut NATIONALLICENCE 700 1- Okamoto Ken-ichi The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut NATIONALLICENCE 700 1- Hasegawa Masami The Institute of Statistical Mathematics 4-6-7 Minami-Azabu, Minato-ku, Tokyo 106 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 70-79 0021-924X 119:1<70 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford