caa a22 4500 397555768 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021207 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021207 DNA Polymerase βs from Liver and Testes of Cherry Salmon, Oncorhynchus masou: Purification and Characterization of DNA Polymerase βs with Acidic Isoelectric Points [Elektronische Daten] [Toyofumi Yamaguchi, Shigeko Nishimura, Keiko Takahashi, Michiyasu Yoshikuni, Junya Masaki, Toshiaki Hirai, Mineo Saneyoshi] DNA polymerase βs from cherry salmon, Oncorhynchus masou, liver and testes were purified to near homogeneity, and no substantial differences between the enzymes were observed. The molecular weight of both enzymes, determined by SDS-polyacrylamide gel electrophoresis, was 39,000. The amino acid sequences of the N-terminus of the liver and testes enzymes were determined and compared with that of the rat enzyme. Of the N-terminal 30 amino acid residues of salmon liver DNA polymerase β,21 (70%) were identical to those of the rat enzyme sequence. However, unlike most eukaryotic DNA polymerase βs, the isoelectric points (pis) of the DNA polymerase βs from salmon liver and testes were both estimated to be 6.2, which is significantly different from the alkaline isoelectric points (pI=8.5-9.5) established for other highly purified vertebrate DNA polymerase βs.The cherry salmon DNA polymerase βs were still active at below 10°C, compared with the rat enzyme. © by the Journal of Biochemistry Regular Papers nationallicence cherry salmon nationallicence DNA polymerase β nationallicence isoelectric point nationallicence N-terminal amino acid sequence nationallicence vertebrates nationallicence Yamaguchi Toyofumi Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut Nishimura Shigeko Shizuoka Higashi Hospital, National Epilepsy Center 886 Urushiyama, Shizuoka 420 aut Takahashi Keiko Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut Yoshikuni Michiyasu Department of Developmental Biology, National Institute for Basic Biology Okazaki, Aichi 444 aut Masaki Junya Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut Hirai Toshiaki Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut Saneyoshi Mineo Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 186-192 0021-924X 119:1<186 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021207 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021207 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Toyofumi Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut NATIONALLICENCE 700 1- Nishimura Shigeko Shizuoka Higashi Hospital, National Epilepsy Center 886 Urushiyama, Shizuoka 420 aut NATIONALLICENCE 700 1- Takahashi Keiko Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut NATIONALLICENCE 700 1- Yoshikuni Michiyasu Department of Developmental Biology, National Institute for Basic Biology Okazaki, Aichi 444 aut NATIONALLICENCE 700 1- Masaki Junya Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut NATIONALLICENCE 700 1- Hirai Toshiaki Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut NATIONALLICENCE 700 1- Saneyoshi Mineo Department of Biological Sciences, The Nishi-Tokyo University Uenohara, Yamanashi 409-01 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 186-192 0021-924X 119:1<186 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford