caa a22 4500 397555792 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021195 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021195 Synthesis and Properties of Dimeric Glycerophospholipid: 2,2′:-Dotriacontanedioylbis(l-Palmitoyl-sn-Glycero-3-Phosphocholine) [Elektronische Daten] [Kiyoshi Yamauchi, Katsuya Togawa, Masayoshi Kinoshita] 2,2′:-Dotriacontanedioylbi8(l-palmitoyl-sn-glycero-3-phosphocholine) was synthesized chemically as a dimeric version of l,2-dipalmitoyl-8n-glycero-3-phosphocholine. Upon sonication in water, the bipolar lipid furnished a planar membrane of about 50 Å thickness with gel-to-liquid crystalline phase transition constants of Tm =53.4°C, ΔH=20.9 kcal/mol, and ΔS = 64.0 e.u. In an air/water interface, the lipid displayed unique surface pressure-surface area isotherms with a limiting area of 105-190 Å/molecule at 10-20°C. The lipid was a substrate of phospholipase A2 (Naja mocambique), which afforded 1-palmitoyl-8n-glycero-3-phosphocholine and dotriacontanedioic acid as final products. © by the Journal of Biochemistry Regular Papers nationallicence gel-to-liquid crystalline phase transition nationallicence lipid membrane nationallicence liposome nationallicence membrane morphology nationallicence phospholipase A2 nationallicence Yamauchi Kiyoshi Department of Bioapplied Chemistry, Osaka City University, Sumiyoshi-ku, Osaka 558 aut Togawa Katsuya Department of Bioapplied Chemistry, Osaka City University, Sumiyoshi-ku, Osaka 558 aut Kinoshita Masayoshi Department of Bioapplied Chemistry, Osaka City University, Sumiyoshi-ku, Osaka 558 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 115-119 0021-924X 119:1<115 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021195 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021195 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamauchi Kiyoshi Department of Bioapplied Chemistry, Osaka City University, Sumiyoshi-ku, Osaka 558 aut NATIONALLICENCE 700 1- Togawa Katsuya Department of Bioapplied Chemistry, Osaka City University, Sumiyoshi-ku, Osaka 558 aut NATIONALLICENCE 700 1- Kinoshita Masayoshi Department of Bioapplied Chemistry, Osaka City University, Sumiyoshi-ku, Osaka 558 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 115-119 0021-924X 119:1<115 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford