caa a22 4500 397555806 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021201 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021201 Stabilization of Lysozyme by Introducing N-Glycosylation Signal Sequence [Elektronische Daten] [Tadashi Ueda, Hiroki Iwashita, Yoshio Hashimoto, Taiji Imoto] We designed mutant lysozymes with N-glycosylation signal sequences (Asn48 -Gly49-Thr-50 and Asn87-Ile88-Thr89) by substituting Asp to Asn at positions 48 and 87. When these mutant lysozymes were expressed by using yeast (Saccharomyces cerevisiae) in Burkhol-der minimum medium, N-glycosylation occurred in both lysozymes. The mutant lysozyme with the oligosaccharide at Asn87 showed a similar character to a reported polymannosyl lysozyme [Nakamura,Takasaki,Kobayashi, and Kato (1993) J. Biol. Chem. 268, 12706-12712; Kato, Takasaki,and Ban (1994) FEBS Lett 355, 76-80]. As judged from the thermodynamic stabilities of the lysozymes obtained by the guanidine hydrochloride denaturation method, the oligosaccharide-bearing mutant lysozymes were more stable by 0.4-1.6 kcal/mol than the corresponding unglycosylated lysozymes. Therefore, it is suggested that the introduction of an N-glycosylation signal sequence into a protein is an effective means to increase the stability of the protein. © by the Journal of Biochemistry Regular Papers nationallicence N-glycosylation signal nationallicence lysozyme nationallicence stabilization nationallicence Ueda Tadashi Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut Iwashita Hiroki Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut Hashimoto Yoshio Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut Imoto Taiji Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 157-161 0021-924X 119:1<157 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021201 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021201 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ueda Tadashi Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut NATIONALLICENCE 700 1- Iwashita Hiroki Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut NATIONALLICENCE 700 1- Hashimoto Yoshio Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut NATIONALLICENCE 700 1- Imoto Taiji Graduate School of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812-82 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 157-161 0021-924X 119:1<157 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford