caa a22 4500 397555857 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021215 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021215 Crystal Structure of H2-Proteinase from the Venom of Trimeresurus flavoviridis [Elektronische Daten] [Takashi Kumasaka, Masaki Yamamoto, Hideaki Moriyama, Nobuo Tanaka, Mamoru Sato, Yukiteru Katsube, Yoshio Yamakawa, Tamotsu Omori-Satoh, Sadaaki Iwanaga, Tatzuo Ueki] The crystal structure of the zinc-protease, H2-proteinase, isolated from the venom of Trimeresurus flavoviridis has been determined. The crystallographic R factor is 0.176 for 10,635 reflections with Fobs>2ρ(Fobs) in the 8.0 to 2.2 Åresolution range. The enzyme has two domains with a cleft in which a catalytic zinc atom is located. The N-terminal domain is composed of four helices around a central five-stranded β-sheet. The irregularly folded C-terminal domain contains one helix and two disulfide bridges. These two domains are linked by a disulfide bridge. In the zinc environment, the catalytic zinc atom forms a distorted tetrahedral coordination with three histidines and one catalytic water molecule, and the methionine-containing turn is structurally conserved. These are distinctive features of the metzincins, one of the zinc metalloprotease superfamilies. The entire structure shows good agreement with that of two Crotalus snake venom proteases, adamalysin II and atrolysin C. The H2-proteinase, however, contains no structural calcium ions, and differences of disulfide configurations and the coordination of the catalytic water molecule exist as compared with the other two proteases. © by the Journal of Biochemistry Regular Papers nationallicence crystal structure nationallicence snake venom nationallicence zinc-protease nationallicence Kumasaka Takashi Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Midori-ku, Yokohama 226 aut Yamamoto Masaki Biophysics Laboratory, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01 aut Moriyama Hideaki Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Midori-ku, Yokohama 226 aut Tanaka Nobuo Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Midori-ku, Yokohama 226 aut Sato Mamoru Institute for Protein Research, Osaka University Suita, Osaka, 565 aut Katsube Yukiteru Institute for Protein Research, Osaka University Suita, Osaka, 565 aut Yamakawa Yoshio Department of Biochemistry and Cell Biology, National Institute of Health Shinjuku-ku, Tokyo 162 aut Omori-Satoh Tamotsu Department of Biochemistry and Cell Biology, National Institute of Health Shinjuku-ku, Tokyo 162 aut Iwanaga Sadaaki Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut Ueki Tatzuo Biophysics Laboratory, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 49-57 0021-924X 119:1<49 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021215 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021215 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumasaka Takashi Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Yamamoto Masaki Biophysics Laboratory, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01 aut NATIONALLICENCE 700 1- Moriyama Hideaki Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Tanaka Nobuo Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Sato Mamoru Institute for Protein Research, Osaka University Suita, Osaka, 565 aut NATIONALLICENCE 700 1- Katsube Yukiteru Institute for Protein Research, Osaka University Suita, Osaka, 565 aut NATIONALLICENCE 700 1- Yamakawa Yoshio Department of Biochemistry and Cell Biology, National Institute of Health Shinjuku-ku, Tokyo 162 aut NATIONALLICENCE 700 1- Omori-Satoh Tamotsu Department of Biochemistry and Cell Biology, National Institute of Health Shinjuku-ku, Tokyo 162 aut NATIONALLICENCE 700 1- Iwanaga Sadaaki Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 700 1- Ueki Tatzuo Biophysics Laboratory, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 49-57 0021-924X 119:1<49 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford