caa a22 4500 397555865 CHVBK 20180308164801.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021206 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021206 NMR of All-Carbon-13 Sugars: An Application in Development of an Analytical Method for a Novel Natural Sugar, 1,5-Anhydrofructose [Elektronische Daten] [Shunichi Kametani, Hideaki Mizuno, Yoko Shiga, Hiroshi Akanuma] Of the all-carbon-13 compounds, glucose is one of the most easily accessible, and therefore we applied 13C-NMR technique to the metabolic study of glucose-related compounds, 1,5-anhydro-D-glucitol and 1,5-anhydro-D-fructose (AF). Applying an INADEQUATE method to the substitutes of these novel sugars fully labeled with carbon-13, we could trace out the entire carbon skeleton with high sensitivity and confirm the chemical structures of these sugars. The method also provided a much easier way to optimize the enzymatic oxidation for AF preparation: we selectively and continuously monitored the quantities, as well as their structures in aqueous solution, of the substrate and products in a noninvasive manner. Similarly relying upon information from the 13C-NME, we developed a valuable derivatization method of AF for its GC-MS application, which was so sensitive that we were able to demonstrate the natural occurrence of AF in rat liver. © by the Journal of Biochemistry Regular Papers nationallicence 1,5-anhydro-D-glucitol nationallicence 1,5-anhydro-D-fructose nationallicence 13C-NMB nationallicence GC-MS nationallicence polyol nationallicence Kametani Shunichi Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut Mizuno Hideaki Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut Shiga Yoko Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut Akanuma Hiroshi Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 180-185 0021-924X 119:1<180 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021206 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021206 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kametani Shunichi Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Mizuno Hideaki Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Shiga Yoko Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Akanuma Hiroshi Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 180-185 0021-924X 119:1<180 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford