caa a22 4500 397555911 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021219 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021219 Nucleotide Sequence of the Uricase Gene from Bacillus sp. TB-90 [Elektronische Daten] [Kazumi Yamamoto, Yoshio Kojima, Toshiro Kikuchi, Tatsuro Shigyo, Kohji Sugihara, Masachika Takashio, Shigenori Emi] The nucleotide sequence of the uricase gene from the thermophilic bacterium Bacillus sp. TB-90 was determined. The primary structure of the uricase deduced from the nucleotide sequence comprised 332 amino acids, with a total molecular mass of 37,994 Da. The molecular mass of the subunit of the uricase produced by the transformant of Escherichia coli agreed well with this value. However, the molecular mass of a subunit of the uricase produced by Bacillus sp. TB-90 was found to be 34,000 Da by SDS-PAGE. The difference between these molecular masses was attributed to processing of the C-terminal 13 amino acid residue in Bacillus sp. TB-90. Comparison of the enzymatic properties of both uricases showed that the thennostability of the uricase produced by the transformant was enhanced by about 10deg;C in comparison to that produced by Bacillus sp. TB-90. © by the Journal of Biochemistry Regular Papers nationallicence Bacillus sp nationallicence nucleotide sequence nationallicence processing C-terminus nationallicence thermostability nationallicence uricase nationallicence Yamamoto Kazumi Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut Kojima Yoshio Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut Kikuchi Toshiro Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut Shigyo Tatsuro Pharmaceutical Research Laboratories, Sapporo Breweries Ltd. 10 Okatohme, Yaizu, Shizuoka 425 aut Sugihara Kohji Pharmaceutical Research Laboratories, Sapporo Breweries Ltd. 10 Okatohme, Yaizu, Shizuoka 425 aut Takashio Masachika Pharmaceutical Research Laboratories, Sapporo Breweries Ltd. 10 Okatohme, Yaizu, Shizuoka 425 aut Emi Shigenori Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 80-84 0021-924X 119:1<80 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021219 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021219 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Kazumi Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut NATIONALLICENCE 700 1- Kojima Yoshio Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut NATIONALLICENCE 700 1- Kikuchi Toshiro Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut NATIONALLICENCE 700 1- Shigyo Tatsuro Pharmaceutical Research Laboratories, Sapporo Breweries Ltd. 10 Okatohme, Yaizu, Shizuoka 425 aut NATIONALLICENCE 700 1- Sugihara Kohji Pharmaceutical Research Laboratories, Sapporo Breweries Ltd. 10 Okatohme, Yaizu, Shizuoka 425 aut NATIONALLICENCE 700 1- Takashio Masachika Pharmaceutical Research Laboratories, Sapporo Breweries Ltd. 10 Okatohme, Yaizu, Shizuoka 425 aut NATIONALLICENCE 700 1- Emi Shigenori Tsuruga Institute of Biotechnology, Toyobo Co., Ltd. 10-24 Toyo-cho, Tsuruga, Fukui 914 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 80-84 0021-924X 119:1<80 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford