caa a22 4500 397555938 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021222 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021222 Release of Spectrin-Containing Vesicles from Human Erythrocyte Ghosts by Dimyristoylphosphatidylcholine [Elektronische Daten] [Takeo Yamaguchi, Misuzu Yamamoto, Eiji Kimoto] Membrane vesicles, which were released from human erythrocyte ghosts by dimyristoylphosphatidylcholine (DMPC), showed a protein composition similar to that of the erythrocyte membrane, despite a reduction of in spectrin content. The spectrin content of vesicles decreased with increasing hemoglobin concentration within ghost membranes, but increased upon exposure of hemoglobin-free ghosts to a pressure of 100 MPa. The ESR spectra of spin-labeled membrane proteins showed that membrane proteins in ghosts became unfolded at high pressure. Furthermore, spectrin-poor and protein 4.1-rich vesicles were released by DMPC from diamide-treated ghosts in which spectrin was cross-linked and stabilized. Taking into account that the spectrin tetramer is stabilized by hemoglobin [Liu and Palek (1984) J. Biol. Chem. 259,11566-11562], these results suggest that the spectrin content of DMPC-induced vesicles from erythrocyte ghosts increases with increasing instability of the cytoskeletal network in parent cells. © by the Journal of Biochemistry Regular Papers nationallicence dimyristoylphosphatidylcholine nationallicence erythrocyte nationallicence hemoglobin nationallicence spectrin nationallicence vesicula-tion nationallicence Yamaguchi Takeo Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-80 aut Yamamoto Misuzu Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-80 aut Kimoto Eiji Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-80 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 95-99 0021-924X 119:1<95 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021222 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021222 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Takeo Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-80 aut NATIONALLICENCE 700 1- Yamamoto Misuzu Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-80 aut NATIONALLICENCE 700 1- Kimoto Eiji Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-80 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 95-99 0021-924X 119:1<95 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford