caa a22 4500 397555962 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021211 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021211 Suppression of Superoxide-Generating Ability during Differentiation of Monocytes to Dendritic Cells [Elektronische Daten] [D. Yu, S. Imajoh-Ohmi, K. Akagawa, S. Kanegasaki] Human peripheral monocytes cultured with GM-CSF and IL-4 differentiated to dendritic cells (DCs) and with GM-CSF alone to macrophages. Superoxide-generating ability in such DCs was found to be suppressed whereas that in macrophages remained constant. To examine the reason for the suppression in DCs, we evaluated by immunoblotting the levels of essential components of the superoxide generating system in the cells during the differentiation. In contrast to the levels of cytosolic 47- and 65-kDa components and Rac-p21, which remained constant throughout cultivation, those of the large and the small subunits of cytochrome b558 were found to decrease quickly by day 2 during cultivation of monocytes with GM-CSF and IL-4. DCs obtained after 7 days of cultivation had lost the large subunit almost completely and most of the small subunit. A cell surface epitope of the cytochrome detected by a monoclonal antibody also decreased during the differentiation. On the other hand, these components, including both subunits of cytochrome b558, were maintained in the cells during differentiation of monocytes to macrophages. These results indicate that the decreased levels of cytochrome b558, especially that of the large subunit, is responsible for the low level of superoxide-generating ability of DCs and that the suppression is caused by IL-4. © by the Journal of Biochemistry Regular Papers nationallicence dendritic cells nationallicence GM-CSF nationallicence IL-4 nationallicence monocytes nationallicence superoxide-generating ability nationallicence Yu D. Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108 aut Imajoh-Ohmi S. Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108 aut Akagawa K. National Institute of Health Japan Tokyo aut Kanegasaki S. Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 23-28 0021-924X 119:1<23 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021211 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021211 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu D. Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108 aut NATIONALLICENCE 700 1- Imajoh-Ohmi S. Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108 aut NATIONALLICENCE 700 1- Akagawa K. National Institute of Health Japan Tokyo aut NATIONALLICENCE 700 1- Kanegasaki S. Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 23-28 0021-924X 119:1<23 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford