caa a22 4500 397556004 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021194 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021194 Extracellular Matrix 22-kDa Protein Interacts with Decorin Core Protein and Is Expressed in Cutaneous Fibrosis [Elektronische Daten] [Osamu Okamoto, Yutaka Suzuki, Sadao Kimura, Hiroshi Shinkai] A protein with an Mr of 22,000 was purified from bovine dermis. The amino acid sequence of the protein was identical to 22-kDa protein [Neame, P.J. et al. (1989) J. BioL Chem. 264, 5474-5479] and showed highly homologous sequences to TRAMP (tyrosine rich acidic matrix protein) [Cronshaw, A.D. et al. (1993) Matrix 13, 255-266] and dermatopontin [Superti-Furga, A. et al. (1993) Genomics 17,463-467]. The protein was proved to associate with decorin and a modified decorin with carboxymethylated cysteinyl residues, but not to assemble to hyaluronate or dennatan sulfate chains. The pyridylethylation of cysteinyl residues in the 22-kDa protein did not affect its binding activity to decorin or modified decorin. Immunohistochemical analyses revealed positive stains in endothelial cells and the periphery of collagen fibers in normal dermis but not in the fibroblasts in tissue. Collagen fibers in sclerotic regions of progressive systemic sclerosis were stained diffusely, suggesting that the 22-kDa protein increases in parallel to the accumulation of collagen in the disease. Western blotting analyses of extracts of cultured endothelial cells revealed a lower Mr protein than that from cultured fibroblasts, suggesting the presence of a molecule related to the 22-kDa protein. © by the Journal of Biochemistry Regular Papers nationallicence decorin nationallicence dermatopontin nationallicence PSS nationallicence TRAMP nationallicence 22 kDa protein nationallicence Okamoto Osamu Department of Dermatology, Oita Medical University Hasama-cho, Oita-gun, Oita 879-56 aut Suzuki Yutaka Department of Dermatology Inohana, Chuo-ku, Chiba 260 aut Kimura Sadao Center for Biomedical Science, Chiba University School of Medicine Inohana, Chuo-ku, Chiba 260 aut Shinkai Hiroshi Department of Dermatology Inohana, Chuo-ku, Chiba 260 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 106-114 0021-924X 119:1<106 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021194 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021194 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okamoto Osamu Department of Dermatology, Oita Medical University Hasama-cho, Oita-gun, Oita 879-56 aut NATIONALLICENCE 700 1- Suzuki Yutaka Department of Dermatology Inohana, Chuo-ku, Chiba 260 aut NATIONALLICENCE 700 1- Kimura Sadao Center for Biomedical Science, Chiba University School of Medicine Inohana, Chuo-ku, Chiba 260 aut NATIONALLICENCE 700 1- Shinkai Hiroshi Department of Dermatology Inohana, Chuo-ku, Chiba 260 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 106-114 0021-924X 119:1<106 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford