caa a22 4500 397556047 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021203 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021203 Relationship between the Concentrations of Glycolytic Intermediates and Expression of the L-Type Pyruvate Kinase Gene in Cultured Hepatocytes [Elektronische Daten] [Rujun Kang, Kazuya Yamada, Takashi Tanaka, Ting Lu, Tamio Noguchi] Previous studies have suggested that some glycolytic intermediates are involved in the regulation of L-type pyruvate kinase gene expression by carbohydrates such as glucose and fructose. To find such intermediates, we examined the relationship between the levels of L-type pyruvate kinase mRNA and glycolytic metabolites in hepatocytes cultured under various conditions. Of the metabolites, the levels of 3-phosphoglycerate and phosphoenol-pyruvate only increased significantly under conditions under which the expression of the L-type pyruvate kinase gene was stimulated. The level of glucose 6-phosphate, which was reported to be involved in dietary stimulation of this gene expression, was not correlated with the mRNA level since marked accumulation of deoxyglucose 6-phosphate occurring on the addition of deoxyglucose, a nonmetabolizable glucose analog, was not accompanied by an increase in the L-type pyruvate kinase mRNA level. In addition, we found that fructose at a low concentration in the presence of glucose failed to increase the mRNA and metabolite levels in contrast to other reports that the promoter activity of the L-type pyruvate kinase gene is stimulated by this treatment. Thus we propose that 3-phosphoglycerate and/or phosphoenolpyruvate are involved in the carbohydrate regulation of L-type pyruvate kinase gene expression. © by the Journal of Biochemistry Regular Papers nationallicence carbohydrate metabolism nationallicence glycolytic metabolites nationallicence L-type pyruvate kinase gene expression nationallicence Kang Rujun Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut Yamada Kazuya Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut Tanaka Takashi Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut Lu Ting Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut Noguchi Tamio Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 119/1(1996-01), 162-166 0021-924X 119:1<162 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021203 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021203 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang Rujun Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 700 1- Yamada Kazuya Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 700 1- Tanaka Takashi Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 700 1- Lu Ting Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 700 1- Noguchi Tamio Department of Nutrition and Physiological Chemistry, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/1(1996-01), 162-166 0021-924X 119:1<162 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford