caa a22 4500 397556063 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021347 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021347 Histone Hyperacetylation Plays a Role in Augmentation of IL-4-Induced IgE Production in LPS-Stimulated Murine B-Lymphocytes by Sodium Butyrate [Elektronische Daten] [Itaru Yamamoto, Tetsuhiko Matsunaga, Kei Sakata, Yuko Nakamura, Seiichi Doi, Fumie Hanmyou] We previously reported the augmentation by sodium butyrate (NaBu) of IL-4-induced IgE production in LPS-stimulated murine B-lymphocytes. Histone deacetylase inhibition may be involved in the molecular mode of action of NaBu. Thus, this study was accomplished to examine the involvement of histone hyperacetylation in IL-4-induced class switching promotion by NaBu with a specific histone deacetylase inhibitor, trichostatin A (TSA). TSA was found to enhance IL-4-dependent IgE production in a concentration-dependent manner (0.3-30 nM) as did NaBu, although neither compound affected the IgE production in the absence of IL-4. The effect of combined addition of suboptimal concentrations of NaBu and TSA to the culture appeared to be additive, not synergistic. Moreover, the intense of enhancement of IgE production observed by addition of both compounds at optimal concentrations appeared to be equivalent to that obtained with each maximal concentration. Furthermore, enhancement of IgE production by TSA or NaBu was confirmed to be absolutely IL-4 dependent and was not due to the shift of kinetics. On the other hand, various cell cycle inhibitors such as caffeine and theophylline (G1 or G2-arrest), hydroxy-urea (S-arrest), and colchicine (M-arrest), none of which have the ability to inhibit histone deacetylase, were shown to have no effect. These findings suggest that histone hyperacetylation plays an important regulatory role in the modification of IL-4-dependent class switching to Cε in LPS-stimulated murine B-cells by NaBu. © by the Journal of Biochemistry Regular Papers nationallicence histone deacetylase nationallicence IgE nationallicence IL-4 nationallicence sodium butyrate nationallicence trichostatin A nationallicence Yamamoto Itaru Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut Matsunaga Tetsuhiko Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut Sakata Kei Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut Nakamura Yuko Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut Doi Seiichi Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut Hanmyou Fumie Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1056-1061 0021-924X 119:6<1056 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021347 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021347 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Itaru Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut NATIONALLICENCE 700 1- Matsunaga Tetsuhiko Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut NATIONALLICENCE 700 1- Sakata Kei Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut NATIONALLICENCE 700 1- Nakamura Yuko Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut NATIONALLICENCE 700 1- Doi Seiichi Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut NATIONALLICENCE 700 1- Hanmyou Fumie Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1056-1061 0021-924X 119:6<1056 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford