caa a22 4500 397556071 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021364 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021364 Enzyme-Linked Immunoassay for Midkine, and Its Application to Evaluation of Midkine Levels in Developing Mouse Brain and Sera from Patients with Hepatocellular Carcinomas [Elektronische Daten] [Hisako Muramatsu, Xiao-jun Song, Norio Koide, Hajime Hada, Takao Tsuji, Kenji Kadomatsu, Tatsuya Inui, Terutoshi Kimura, Shumpei Sakakibara, Takashi Muramatsu] Midkine (MK) is a growth factor that promotes neurite outgrowth and survival of neurons, and enhances the plasminogen activator in endothelial cells. A highly sensitive enzyme-linked immunoassay for MK was developed, involving affinity-purified anti-MK antibodies, their biotinylated form, and avidin-β-galactosidase. The amount of bound avidin-β-galactosidase was determined using a fluorogenic substrate, 4-methylumbelliferyl-β-D-galactoside. This method allowed the detection of human and mouse MK in the range of 50 pg-10 ng. Pleiotrophin, which is related to MK in its amino acid sequence, did not show any cross reactivity. Employing this method, the MK levels in the developing mouse brain were determined. The MK level was 2 μg/g of wet tissue on the 12th day of gestation, and then steadily decreased during embryogenesis and postnatal development to 30 ng/g two months after birth. The assay method can also be applied to serum samples. Although the MK levels in the sera of normal human subjects were low or undetectable, 0.6-8 ng/ml of MK was detected in samples in the majority of cases of hepatocellular carcinomas. © by the Journal of Biochemistry Regular Papers nationallicence brain development nationallicence enzyme immunoassay nationallicence hepatocellular carcinoma nationallicence midkine nationallicence Muramatsu Hisako Department of Biochemistry, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, 2-5-1 Shikata-cho, Okayama 700, 4-1-2 Ina, Minoh, Osaka 562 aut Song Xiao-jun Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut Koide Norio Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut Hada Hajime Department of Internal Medicine, Okayama University School of Medicine 2-5-1 Shikata-cho, Okayama 700 aut Tsuji Takao Department of Internal Medicine, Okayama University School of Medicine 2-5-1 Shikata-cho, Okayama 700 aut Kadomatsu Kenji Department of Internal Medicine, Okayama University School of Medicine 2-5-1 Shikata-cho, Okayama 700 aut Inui Tatsuya Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut Kimura Terutoshi Peptide Institute Inc. 4-1-2 Ina, Minoh, Osaka 562 aut Sakakibara Shumpei Peptide Institute Inc. 4-1-2 Ina, Minoh, Osaka 562 aut Muramatsu Takashi Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1171-1175 0021-924X 119:6<1171 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021364 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021364 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muramatsu Hisako Department of Biochemistry, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, 2-5-1 Shikata-cho, Okayama 700, 4-1-2 Ina, Minoh, Osaka 562 aut NATIONALLICENCE 700 1- Song Xiao-jun Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut NATIONALLICENCE 700 1- Koide Norio Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut NATIONALLICENCE 700 1- Hada Hajime Department of Internal Medicine, Okayama University School of Medicine 2-5-1 Shikata-cho, Okayama 700 aut NATIONALLICENCE 700 1- Tsuji Takao Department of Internal Medicine, Okayama University School of Medicine 2-5-1 Shikata-cho, Okayama 700 aut NATIONALLICENCE 700 1- Kadomatsu Kenji Department of Internal Medicine, Okayama University School of Medicine 2-5-1 Shikata-cho, Okayama 700 aut NATIONALLICENCE 700 1- Inui Tatsuya Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut NATIONALLICENCE 700 1- Kimura Terutoshi Peptide Institute Inc. 4-1-2 Ina, Minoh, Osaka 562 aut NATIONALLICENCE 700 1- Sakakibara Shumpei Peptide Institute Inc. 4-1-2 Ina, Minoh, Osaka 562 aut NATIONALLICENCE 700 1- Muramatsu Takashi Department of Biochemistry, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1171-1175 0021-924X 119:6<1171 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford