caa a22 4500 397556098 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021351 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021351 Expression of Bombyx Family Fungal Protease Inhibitor F from Bombyx mori by Baculovirus Vector [Elektronische Daten] [Thiên-Ngoc Pham, Kaeko Hayashi, Ryo Takano, Hiroshi Nakazawa, Hajime Mori, Masatoshi Ichida, Masanobu Itoh, Masaharu Eguchi, Fujiyoshi Matsubara, Saburo Hara] Fungal protease inhibitor F (FPI-F) from silkworm hemolymph is a novel serine protease inhibitor of the Bombyx family. The cDNA of FPI-F was introduced into a baculovirus vector and a recombinant virus was isolated and plaque-purified. The protease inhibitory activities increased in the culture medium of insect cells and in the hemolymph of silkworms infected with the recombinant virus. Judged from the behavior on ion-exchange and reversed-phase chromatographies, amino acid compositions, amino-terminal sequences, and CD spectra, the recombinant FPI-F was identical with native FPI-F. Infection with the recombinant virus caused inhibition of larval development of the silkworm. However, the degree of the effect was different in two strains, Shinryukaku and Taiheichoan, indicating that the selection of the strain of silkworm is important in using the baculovirus expression system. © by the Journal of Biochemistry Regular Papers nationallicence baculovirus nationallicence Bombyx family inhibitor nationallicence expression nationallicence serine protease inhibitor nationallicence silkworm nationallicence Pham Thiên-Ngoc Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Hayashi Kaeko Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Takano Ryo Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Nakazawa Hiroshi Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Mori Hajime Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Ichida Masatoshi Experimental Farms, Faculty of Textile Science, Kyoto Institute of Technology Sagaippongi-cho, Ukyo-ku, Kyoto 616 aut Itoh Masanobu Experimental Farms, Faculty of Textile Science, Kyoto Institute of Technology Sagaippongi-cho, Ukyo-ku, Kyoto 616 aut Eguchi Masaharu Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Matsubara Fujiyoshi Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut Hara Saburo Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1080-1085 0021-924X 119:6<1080 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021351 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021351 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pham Thiên-Ngoc Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Hayashi Kaeko Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Takano Ryo Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Nakazawa Hiroshi Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Mori Hajime Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Ichida Masatoshi Experimental Farms, Faculty of Textile Science, Kyoto Institute of Technology Sagaippongi-cho, Ukyo-ku, Kyoto 616 aut NATIONALLICENCE 700 1- Itoh Masanobu Experimental Farms, Faculty of Textile Science, Kyoto Institute of Technology Sagaippongi-cho, Ukyo-ku, Kyoto 616 aut NATIONALLICENCE 700 1- Eguchi Masaharu Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Matsubara Fujiyoshi Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 700 1- Hara Saburo Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1080-1085 0021-924X 119:6<1080 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford