caa a22 4500 397556101 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021362 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021362 Purification and Characterization of a Novel Hyaluronan-Binding Protein (PHBP) from Human Plasma: It Has Three EGF, a Kringle and a Serine Protease Domain, Similar to Hepatocyte Growth Factor Activator [Elektronische Daten] [Nam-Ho Choi-Miura, Takashi Tobe, Jun-ichi Sumiya, Yasuko Nakano, Yoshihiro Sano, Toshio Mazda, Motowo Tomita] A novel hyaluronan-binding protein (PHBP) was purified from human plasma by affinity chromatography on hyaluronan-conjugated Sepharose. The contaminating IgM and albumin in the partially purified preparation were removed with anti-IgG antibody-conjugated Sepharose and anti-albumin antibody-conjugated Sepharose, respectively, and no other contaminant was observed. Finally, 800 μg of PHBP was isolated from 500 ml of human plasma. PHBP gave a single 70-kDa band on SDS-PAGE under non-reducing conditions, and 50-kDa and 17-kDa bands under reducing conditions. Thus, PHBP was a heterodimer composed of 50-kDa and 17-kDa subunits, bridged by a disulfide linkage. Both subunits had novel N-terminal ami no acid sequences, indicating that PHBP was a novel hyaluronan-binding protein in human plasma. The amino acid sequence deduced from the nucleotide sequence of the cloned PHBP cDNA exhibited significant homology to that of hepatocyte growth factor activator (HGFA). The results of Northern blot analysis indicated that liver, kidney, and pancreas expressed PHBP mRNA. The predicted structure of PHBP showed three epidermal growth factor (EGF) domains, a kringle domain and a serine protease domain, from its N-terminus, although HGFA has a fibronectin type H domain, an EGF domain, a fibronectin type I domain, an EGF domain, a kringle domain, and a serine protease domain, from its N-terminus. © by the Journal of Biochemistry Regular Papers nationallicence hepatocyte growth factor nationallicence hyaluronic acid nationallicence macrophage-stimulating protein nationallicence plasma protein nationallicence serine protease nationallicence Choi-Miura Nam-Ho Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142, Musashino, Tokyo 180 aut Tobe Takashi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Sumiya Jun-ichi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Nakano Yasuko Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Sano Yoshihiro Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut Mazda Toshio Japanese Red Cross Tokyo Blood Center Musashino, Tokyo 180 aut Tomita Motowo Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1157-1165 0021-924X 119:6<1157 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021362 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021362 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi-Miura Nam-Ho Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142, Musashino, Tokyo 180 aut NATIONALLICENCE 700 1- Tobe Takashi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Sumiya Jun-ichi Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Nakano Yasuko Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Sano Yoshihiro Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 700 1- Mazda Toshio Japanese Red Cross Tokyo Blood Center Musashino, Tokyo 180 aut NATIONALLICENCE 700 1- Tomita Motowo Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1157-1165 0021-924X 119:6<1157 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford