caa a22 4500 397556136 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021367 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021367 Developmental Changes of Neutral Glycosphingolipids as Receptors for Pulmonary Surfactant Protein SP-A in the Alveolar Epithelium of Murine Lung [Elektronische Daten] [Kanako Momoeda, Keiko Hirota, Tadahito Utsuki, Yoshiaki Tsuchida, Kazuo Hanaoka, Masao Iwamori] A dramatic change in the glycosphingolipid composition in murine lung occurred between 1 day and 1 week after birth. GlcCer and LacCer were the predominant neutral glycosphingolipids prenatally and 1 day after birth, and the concentrations of globo- and ganglio-series glycosphingolipids increased abruptly from 1 week after birth, reaching maxima at 2-3 weeks. To explore the functional significance of the change, we examined the role of neutral glycosphingolipids as receptors for the murine pulmonary surfactant protein, SP-A, and found that SP-A bound to LacCer, Gg3Cer, and Gg4Cer, but not to Gb3Cer, Gb4Cer, IV3GalNAca-Gb4Cer, sulfatide, or several gangliosides. On TLC-blotting with 125I-labeled SP-A, the binding of SP-A to Gg3Cer and Gg4Cer was 5 times higher than that to LacCer, and on immunohistochemical staining Gg4Cer and Gg3Cer was mainly observed in the alveolar epithelium. Thus, the capacity to retain SP-A of glycolipid receptors per gram dry weight of lung at 1 week after birth was 1.6 times higher than that at 1 day after birth, and reached a maximum 3 weeks after birth. These findings suggest that the enhanced synthesis of the ganglio-series neutral glycosphingolipids 1 week after birth results in an increase in the binding capacity for SP-A on the epithelial cell surface of alveoli. © by the Journal of Biochemistry Regular Papers nationallicence blotting assay nationallicence c-type lectin nationallicence gangio-series nationallicence glycolipid nationallicence immunohistochemis-try nationallicence Momoeda Kanako Departments of Anesthesiology The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut Hirota Keiko Departments of Anesthesiology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut Utsuki Tadahito Departments of Pediatric Surgery, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut Tsuchida Yoshiaki Departments of Pediatric Surgery, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut Hanaoka Kazuo Departments of Anesthesiology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut Iwamori Masao Biochemistry, Faculty of Medicine, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1189-1195 0021-924X 119:6<1189 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021367 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021367 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Momoeda Kanako Departments of Anesthesiology The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Hirota Keiko Departments of Anesthesiology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Utsuki Tadahito Departments of Pediatric Surgery, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Tsuchida Yoshiaki Departments of Pediatric Surgery, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Hanaoka Kazuo Departments of Anesthesiology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Iwamori Masao Biochemistry, Faculty of Medicine, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1189-1195 0021-924X 119:6<1189 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford