caa a22 4500 397556144 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021365 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021365 Evidence for the Existence of Ca2+/calmodulin-Dependent Protein Kinase IV Kinase Isoforms in Rat Brain [Elektronische Daten] [Sachiko Okuno, Takako Kitani, Hitoshi Fujisawa] Calmodulin-dependent protein kinase IV (CaM-kinase IV), which plays crucial roles in the functioning of Ca2+ in the central nervous system and immune system, is markedly activated upon phosphorylation by the action of CaM-kinase IV kinase. Northern and Western blot analyses of CaM-kinase IV kinase showed relatively weak reactions in the rat cerebellum, where the activity of CaM-kinase IV kinase has been demonstrated to exist, indicating that CaM-kinase IV kinase isoforms distinct from the enzyme cloned from the cerebral cortex may exist in the cerebellum. When the crude extracts of rat cerebral cortex, brain stem, and cerebellum were immunotitrated with antibody against the cloned enzyme, only approximately 46, 56, and 25% of the enzyme activity of the respective extracts were immunoprecipitated. Thus, at least two distinct isoforms of CaM-kinase IV kinase appear to exist in the brain. © by the Journal of Biochemistry Regular Papers nationallicence brain nationallicence Ca2+ nationallicence /calmodulin nationallicence CaM-kinase IV kinase nationallicence isoform nationallicence protein kinase kinase nationallicence Okuno Sachiko Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido 078 aut Kitani Takako Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido 078 aut Fujisawa Hitoshi Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido 078 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1176-1181 0021-924X 119:6<1176 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021365 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021365 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okuno Sachiko Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido 078 aut NATIONALLICENCE 700 1- Kitani Takako Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido 078 aut NATIONALLICENCE 700 1- Fujisawa Hitoshi Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido 078 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1176-1181 0021-924X 119:6<1176 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford