caa a22 4500 397556160 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021361 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021361 Midkine, a Heparin-Binding Growth/Differentiation Factor, Exhibits Nerve Cell Adhesion and Guidance Activity for Neurite Outgrowth In Vitro [Elektronische Daten] [Norio Kaneda, Amjad H. Talukder, Hiroko Nishiyama, Shinichi Koizumi, Takashi Muramatsu] By means of a baculovirus expression system, a large amount of mouse midkine (MK) was produced. The protein was purified to homogeneity by heparin-Sepharose column chro-matography. The purified protein was of a mature type; the signal peptide was cleaved at the expected site. To examine the neurite-guiding activity of MK, rat embryonic brain cells (embryonic days 17-18) were cultured on plates coated with purified MK in a grid pattern. The cells attached to and extended their neurites along the substrate pattern. This interaction was strongly inhibited by heparin, but not by other glycosaminoglycans. Treatment of the cells with heparitinase was effective for inhibiting their adhesion to the substrate. These data suggest that the heparin-like domain on cell surface heparan sulfate proteoglycan is the primary site for MK binding upon interaction with nerve cells. © by the Journal of Biochemistry Regular Papers nationallicence baculovirus nationallicence guidance molecule nationallicence heparin-binding protein nationallicence midkine nationallicence neurite outgrowth nationallicence Kaneda Norio Department of Biochemistry, Nagoya University School of Medicine Showa-ku, Nagoya 466 aut Talukder Amjad H. Department of Biochemistry, Nagoya University School of Medicine Showa-ku, Nagoya 466 aut Nishiyama Hiroko Bio-Organics Research Department, International Research Laboratories, Ciba-Geigy Japan Ltd. Takarazuka 665 aut Koizumi Shinichi Bio-Organics Research Department, International Research Laboratories, Ciba-Geigy Japan Ltd. Takarazuka 665 aut Muramatsu Takashi Department of Biochemistry, Nagoya University School of Medicine Showa-ku, Nagoya 466 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1150-1156 0021-924X 119:6<1150 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021361 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021361 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaneda Norio Department of Biochemistry, Nagoya University School of Medicine Showa-ku, Nagoya 466 aut NATIONALLICENCE 700 1- Talukder Amjad H. Department of Biochemistry, Nagoya University School of Medicine Showa-ku, Nagoya 466 aut NATIONALLICENCE 700 1- Nishiyama Hiroko Bio-Organics Research Department, International Research Laboratories, Ciba-Geigy Japan Ltd. Takarazuka 665 aut NATIONALLICENCE 700 1- Koizumi Shinichi Bio-Organics Research Department, International Research Laboratories, Ciba-Geigy Japan Ltd. Takarazuka 665 aut NATIONALLICENCE 700 1- Muramatsu Takashi Department of Biochemistry, Nagoya University School of Medicine Showa-ku, Nagoya 466 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1150-1156 0021-924X 119:6<1150 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford